一个顺式编码的 sRNA 控制着 Yersinia 中 fabH2 的表达。
A cis-encoded sRNA controls the expression of fabH2 in Yersinia.
机构信息
Key Laboratory of Etiology and Biosafety for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
Department of Molecular Biology and Umeå Centre for Microbial Research (UCMR), Umeå University, SE-901 87 Umeå, Sweden.
出版信息
FEBS Lett. 2014 May 21;588(10):1961-6. doi: 10.1016/j.febslet.2014.04.005. Epub 2014 Apr 13.
YsrH is a novel cis-encoded sRNA located on the opposite strand to fabH2, which is essential for fatty acid biosynthesis in bacteria. In this study, YsrH-mediated regulation of fabH2 expression was investigated in Yersinia pseudotuberculosis. Constitutive and inducible over-expression of YsrH decreased the mRNA level of fabH2, while expression of downstream fabD and fabG remained unaffected. Polynucleotide phosphorylase (PNPase) also played an important role in this regulation process by mediating YsrH decay in the exponential phase. Thus, our data defines a cis-encoded sRNA that regulates fatty acid synthesis via a regulatory mechanism also involving PNPase.
YsrH 是一种新型的顺式编码的 sRNA，位于 fabH2 的互补链上，对于细菌脂肪酸的生物合成至关重要。在本研究中，我们研究了 YsrH 对假结核耶尔森氏菌 fabH2 表达的调控作用。YsrH 的组成型和诱导型过表达降低了 fabH2 的 mRNA 水平，而下游 fabD 和 fabG 的表达不受影响。多核苷酸磷酸化酶（PNPase）也通过介导指数期 YsrH 的衰减在这个调控过程中发挥了重要作用。因此，我们的数据定义了一种顺式编码的 sRNA，它通过一种也涉及 PNPase 的调控机制来调节脂肪酸的合成。
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