[全自动免疫组织化学法检测非小细胞肺癌中的ALK融合基因]
[ALK fusion gene assessment by fully automatic immunohistochemistry in non-small cell lung cancer].
作者信息
Guo Lei, Liu Xiuyun, Qiu Tian, Ling Yun, Shan Ling, Xie Yongqiang
机构信息
Department of Pathology, Cancer Institute & Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China.
Department of Pathology, Cancer Institute & Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China. E-mail:
出版信息
Zhonghua Bing Li Xue Za Zhi. 2014 Feb;43(2):95-8.
OBJECTIVE
To evaluate the sensitivity and specificity of fully automated immunohistochemistry (IHC), with comparison to FISH, in the detection of EML4-ALK rearrangement in lung adenocarcinoma (ADC); and the use of IHC as a pre-screening tool.
METHODS
A total of 404 paraffin-embedded NSCLC samples from surgical resections were tested by IHC with Ventana anti-ALK rabbit monoclonal antibody (D5F3) and ultrasensitive detection kit. ALK rearrangement was further confirmed by FISH.
RESULTS
Twenty-nine of 404 lung ADCs (7.2%) were positive for ALK by IHC. ALK positive tumor cells demonstrated strong and diffused granular cytoplasmic staining. All the ALK IHC-positive cases were confirmed to harbor ALK rearrangement by FISH. None of the ALK IHC-negative cases was FISH-positive.
CONCLUSIONS
IHC can effectively detect ALK rearrangement in lung cancer. It may provide a reliable and cost-effective diagnostic approach in routine pathologic laboratories for the identification of suitable candidates for ALK-targeted therapy.
目的
评估全自动免疫组织化学（IHC）在检测肺腺癌（ADC）中EML4-ALK重排方面的敏感性和特异性，并与荧光原位杂交（FISH）进行比较；以及评估IHC作为一种预筛查工具的应用。
方法
使用Ventana抗ALK兔单克隆抗体（D5F3）和超敏检测试剂盒，对404例手术切除的石蜡包埋非小细胞肺癌（NSCLC）样本进行IHC检测。通过FISH进一步确认ALK重排。
结果
404例肺ADC中，29例（7.2%）通过IHC检测ALK呈阳性。ALK阳性肿瘤细胞表现为强烈弥漫的颗粒状细胞质染色。所有IHC检测ALK阳性的病例经FISH确认均存在ALK重排。所有IHC检测ALK阴性的病例FISH检测均为阴性。
结论
IHC能够有效检测肺癌中的ALK重排。它可为常规病理实验室提供一种可靠且经济高效的诊断方法，用于识别适合接受ALK靶向治疗的患者。
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