Dubick M A, Yu G S, Majumdar A P
Division of Military Trauma Research, Letterman Army Institute of Research, Presidio of San Francisco, CA 94129.
J Nutr. 1989 Aug;119(8):1165-72. doi: 10.1093/jn/119.8.1165.
Earlier histological studies have demonstrated that copper deficiency results in a selective and progressive atrophy of pancreatic acinar tissue. The present study examined both biochemical and morphological changes of the exocrine pancreas in nutritional copper deficiency. Groups of mature female rats were fed a purified diet either deficient (less than 0.5 micrograms/g) or sufficient (6.2 micrograms/g) in copper for 6 wk. Copper deficiency resulted in distinct ultrastructural changes in acinar cells, including marked variability in zymogen granule content, autophagic vacuoles and dilation of acinar lumen. Pancreatic weight and total DNA, RNA and protein content of the pancreas were similar in both groups of rats, whereas pancreatic amylase, trypsin and chymotrypsin activity was significantly lower in the copper-deficient group. In addition, secretagogue-induced release of these enzymes from dispersed acini isolated from copper-deficient rats was significantly reduced in comparison to enzyme secretion from normal controls. Pancreatic Cu-Zn and Mn superoxide dismutase activity was also found to be significantly lower in the copper-deficient rats than in normal controls. We conclude that nutritional copper deficiency in adult female rats reduces the responsiveness of the pancreas to secretagogues and may increase the susceptibility of the pancreas to oxidative damage.
早期的组织学研究表明，铜缺乏会导致胰腺腺泡组织选择性和进行性萎缩。本研究检测了营养性铜缺乏时外分泌胰腺的生化和形态学变化。将成年雌性大鼠分组，分别给予铜含量不足（低于0.5微克/克）或充足（6.2微克/克）的纯化饮食6周。铜缺乏导致腺泡细胞出现明显的超微结构变化，包括酶原颗粒含量显著变化、自噬空泡以及腺泡腔扩张。两组大鼠的胰腺重量以及胰腺的总DNA、RNA和蛋白质含量相似，而铜缺乏组的胰腺淀粉酶、胰蛋白酶和糜蛋白酶活性显著降低。此外，与正常对照组的酶分泌相比，从铜缺乏大鼠分离的分散腺泡中，促分泌剂诱导的这些酶的释放显著减少。还发现铜缺乏大鼠的胰腺铜锌和锰超氧化物歧化酶活性明显低于正常对照组。我们得出结论，成年雌性大鼠的营养性铜缺乏会降低胰腺对促分泌剂的反应性，并可能增加胰腺对氧化损伤的易感性。
