Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia.
Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia.
Diagn Microbiol Infect Dis. 2014 Jul;79(3):317-21. doi: 10.1016/j.diagmicrobio.2014.03.017. Epub 2014 Mar 22.
The current study examined the efficacy of the RIDAGENE norovirus (NoV) real-time polymerase chain reaction assay (R-Biopharm, Darmstadt, Germany) for use in a routine diagnostic laboratory. The RIDAGENE assay had an overall sensitivity of 98% but was more sensitive for GII than GI NoV. The assay had a specificity of 98%. The RIDAGENE assay could detect a variety of GI and GII open reading frame 2 genotypes including GI.1, GI.3, GI.8, GI.13, GII.2, GII.3, GII.4 (including the following variants: 2006b, 2009, 2012, and 3 others that have not been assigned), GII.6, GII.12, and GII.13. The assay did not cross react with a number of gastroenteritis viruses including adenovirus, astrovirus, rotavirus, and sapovirus. The assay was straightforward to perform, and for a run of 50 specimens, a result was obtainable in roughly 4 hours. The RIDAGENE assay can be recommended as a valuable detection method for NoV.
本研究旨在评估 RIDAGENE 诺如病毒（NoV）实时聚合酶链反应检测试剂盒（R-Biopharm，达姆施塔特，德国）在常规诊断实验室中的应用效果。RIDAGENE 检测试剂盒的整体敏感性为 98%，但对 GII 型 NoV 的检测更为敏感。该检测试剂盒的特异性为 98%。RIDAGENE 检测试剂盒可检测多种 GI 和 GII 开放阅读框 2 基因型，包括 GI.1、GI.3、GI.8、GI.13、GII.2、GII.3、GII.4（包括以下变体：2006b、2009、2012 和其他 3 种尚未确定的变体）、GII.6、GII.12 和 GII.13。该检测试剂盒与多种胃肠炎病毒（包括腺病毒、星状病毒、轮状病毒和杯状病毒）无交叉反应。该检测试剂盒操作简便，对 50 个样本的检测，大约可在 4 小时内得到结果。RIDAGENE 检测试剂盒可作为一种有价值的 NoV 检测方法。
