哺乳动物细胞中的CRISPR转录抑制装置及分层回路
CRISPR transcriptional repression devices and layered circuits in mammalian cells.
作者信息
Kiani Samira, Beal Jacob, Ebrahimkhani Mohammad R, Huh Jin, Hall Richard N, Xie Zhen, Li Yinqing, Weiss Ron
机构信息
1] Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Raytheon BBN Technologies, Cambridge, Massachusetts, USA.
出版信息
Nat Methods. 2014 Jul;11(7):723-6. doi: 10.1038/nmeth.2969. Epub 2014 May 5.
Abstract
A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.
摘要
构建复杂基因电路的一个关键障碍是缺乏可扩展的器件库。在此，我们提出了一种基于成簇规律间隔短回文重复序列（CRISPR）系统的模块化转录抑制架构，并研究了在人类细胞中调控导向RNA表达的方法。随后，我们证明CRISPR调控器件可以分层构建功能性级联电路，这为工程应用提供了一个有价值的工具箱。
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