通过囊泡中肌动蛋白皮层的自组装来模拟细胞皮层的机械特性。
Mimicking the mechanical properties of the cell cortex by the self-assembly of an actin cortex in vesicles.
作者信息
Luo Tianzhi, Srivastava Vasudha, Ren Yixin, Robinson Douglas N
机构信息
Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA.
Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA ; Department of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA.
出版信息
Appl Phys Lett. 2014 Apr 14;104(15):153701. doi: 10.1063/1.4871861. Epub 2014 Apr 17.
The composite of the actin cytoskeleton and plasma membrane plays important roles in many biological events. Here, we employed the emulsion method to synthesize artificial cells with biomimetic actin cortex in vesicles and characterized their mechanical properties. We demonstrated that the emulsion method provides the flexibility to adjust the lipid composition and protein concentrations in artificial cells to achieve the desired size distribution, internal microstructure, and mechanical properties. Moreover, comparison of the cortical elasticity measured for reconstituted artificial cells to that of real cells, including those manipulated using genetic depletion and pharmacological inhibition, strongly supports that actin cytoskeletal proteins are dominant over lipid molecules in cortical mechanics. Our study indicates that the assembly of biological systems in artificial cells with purified cellular components provides a powerful way to answer biological questions.
肌动蛋白细胞骨架与质膜的复合体在许多生物学过程中发挥着重要作用。在此，我们采用乳液法在囊泡中合成具有仿生肌动蛋白皮层的人工细胞，并对其力学性质进行了表征。我们证明，乳液法能够灵活调整人工细胞中的脂质组成和蛋白质浓度，以实现所需的尺寸分布、内部微观结构和力学性质。此外，将重构人工细胞与真实细胞（包括通过基因敲除和药物抑制处理的细胞）的皮层弹性进行比较，有力地支持了在皮层力学中肌动蛋白细胞骨架蛋白比脂质分子更具主导性。我们的研究表明，用纯化的细胞成分在人工细胞中构建生物系统为解答生物学问题提供了一种有力方法。
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