不同铜源对Caco-2细胞中Ctr1、ATP7A、ATP7B、MT和DMT1蛋白及基因表达的影响。

Effects of different sources of copper on Ctr1, ATP7A, ATP7B, MT and DMT1 protein and gene expression in Caco-2 cells.

作者信息

Gao Chen, Zhu Lianqin, Zhu Fenghua, Sun Jinquan, Zhu Zuxian

机构信息

College of Veterinary Medicine, Jilin University, Changchun 130062, China; College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China; College of Agriculture, Dezhou University, Dezhou 253023, China.

College of Veterinary Medicine, Jilin University, Changchun 130062, China; College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.

出版信息

J Trace Elem Med Biol. 2014 Jul;28(3):344-50. doi: 10.1016/j.jtemb.2014.04.004. Epub 2014 Apr 24.

DOI:10.1016/j.jtemb.2014.04.004

PMID:24815816

Abstract

Copper sulfate (CuSO4), micron copper oxide (micron CuO) and nano copper oxide (nano CuO) at different concentrations were, respectively, added to culture media containing Caco-2 cells and their effects on Ctr1, ATP7A/7B, MT and DMT1 gene expression and protein expression were investigated and compared. The results showed that nano CuO promoted mRNA expression of Ctr1 in Caco-2 cells, and the difference was significant compared with micron CuO and CuSO4. Nano CuO was more effective in promoting the expression of Ctr1 protein than CuSO4 and micron CuO at the same concentration. Nano CuO at a concentration of 62.5 μM increased the mRNA expression levels of ATP7A and ATP7B, and the difference was significant compared with CuSO4. The addition of CuSO4 and nano CuO to the culture media promoted the expression of ATP7B proteins. CuSO4 at a concentration of 125 μM increased the mRNA expression level of MT in Caco-2 cells, and the difference was significant compared with nano CuO and micron CuO. Nano CuO at a concentration of 62.5 μM inhibited the mRNA expression of DMT1, and the difference was significant compared with CuSO4 and micron CuO. Thus, the effects of CuSO4, micron CuO and nano CuO on the expression of copper transport proteins and the genes encoding these proteins differed considerably. Nano CuO has a different uptake and transport mechanism in Caco-2 cells to those of CuSO4 and micron CuO.

摘要

将不同浓度的硫酸铜（CuSO₄）、微米级氧化铜（微米 CuO）和纳米级氧化铜（纳米 CuO）分别添加到含有 Caco-2 细胞的培养基中，研究并比较它们对 Ctr1、ATP7A/7B、MT 和 DMT1 基因表达及蛋白质表达的影响。结果表明，纳米 CuO 促进了 Caco-2 细胞中 Ctr1 的 mRNA 表达，与微米 CuO 和 CuSO₄ 相比差异显著。在相同浓度下，纳米 CuO 比 CuSO₄ 和微米 CuO 更有效地促进 Ctr1 蛋白的表达。浓度为 62.5 μM 的纳米 CuO 增加了 ATP7A 和 ATP7B 的 mRNA 表达水平，与 CuSO₄ 相比差异显著。向培养基中添加 CuSO₄ 和纳米 CuO 促进了 ATP7B 蛋白的表达。浓度为 125 μM 的 CuSO₄ 增加了 Caco-2 细胞中 MT 的 mRNA 表达水平，与纳米 CuO 和微米 CuO 相比差异显著。浓度为 62.5 μM 的纳米 CuO 抑制了 DMT1 的 mRNA 表达，与 CuSO₄ 和微米 CuO 相比差异显著。因此，CuSO₄、微米 CuO 和纳米 CuO 对铜转运蛋白及其编码基因表达的影响差异很大。纳米 CuO 在 Caco-2 细胞中的摄取和转运机制与 CuSO₄ 和微米 CuO 不同。