人工关节感染患者中细菌对全髋关节假体不同组件的黏附
Bacterial adherence to different components of total hip prosthesis in patients with prosthetic joint infection.
作者信息
Lass Richard, Giurea Alexander, Kubista Bernd, Hirschl Alexander M, Graninger Wolfgang, Presterl Elisabeth, Windhager Reinhard, Holinka Johannes
机构信息
Department of Orthopedic Surgery, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria,
出版信息
Int Orthop. 2014 Aug;38(8):1597-602. doi: 10.1007/s00264-014-2358-2. Epub 2014 May 10.
Abstract
PURPOSE
The purpose of our study was to evaluate and quantify the bacterial adherence to the different components of total hip prosthesis.
METHODS
The bacterial load of 80 retrieved hip components from 24 patients was evaluated by counting of colony-forming units (CFU) dislodged from component surfaces using the sonication culture method.
RESULTS
Micro-organisms were detected in 68 of 80 explanted components. The highest bacterial load was detected on the polyethylene liners, showing a significant difference in distribution of CFU between the liner and metal components (stem and cup). Staphylococcus epidermidis was identified as the pathogen causing the highest CFU count, especially from the polyethylene liner.
CONCLUSIONS
Results of our study confirm that sonicate culture of the retrieved liners and heads, which revealed the highest bacterial loads, are reliable and sufficient for pathogen detection in the clinical diagnostic routine.
摘要
目的
我们研究的目的是评估和量化细菌对全髋关节假体不同部件的黏附情况。
方法
采用超声培养法，通过计数从部件表面脱落的菌落形成单位（CFU），评估了24例患者80个取出的髋关节部件的细菌载量。
结果
在80个取出的部件中，68个检测到微生物。在聚乙烯衬垫上检测到的细菌载量最高，衬垫与金属部件（柄和髋臼杯）之间的CFU分布存在显著差异。表皮葡萄球菌被确定为导致CFU计数最高的病原体，尤其是来自聚乙烯衬垫的。
结论
我们的研究结果证实，对取出的衬垫和股骨头进行超声培养，其细菌载量最高，在临床诊断常规中对于病原体检测是可靠且充分的。
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