应用抗生素选择标记进行线虫遗传学研究。

Applying antibiotic selection markers for nematode genetics.

机构信息

Cancer and Human Molecular Genetics, Bellvitge Biomedical Research Institute-IDIBELL, Hospitalet de Llobregat, Barcelona 08908, Spain; Univ. Bordeaux, IECB, Laboratoire ARNA, F-33600 Pessac, France; INSERM, U869, Laboratoire ARNA, F-33000 Bordeaux, France.

Univ. Bordeaux, IECB, Laboratoire ARNA, F-33600 Pessac, France; INSERM, U869, Laboratoire ARNA, F-33000 Bordeaux, France.

出版信息

Methods. 2014 Aug 1;68(3):403-8. doi: 10.1016/j.ymeth.2014.04.016. Epub 2014 May 10.

DOI:10.1016/j.ymeth.2014.04.016

PMID:24821108

Abstract

Antibiotic selection markers have been recently developed in the multicellular model organism Caenorhabditis elegans and other related nematode species, opening great opportunities in the field of nematode transgenesis. Here we describe how these antibiotic selection systems can be easily combined with many well-established genetic approaches to study gene function, improving time- and cost-effectiveness of the nematode genetic toolbox.

摘要

抗生素选择标记物最近在多细胞模式生物秀丽隐杆线虫和其他相关线虫物种中得到了开发，为线虫转基因领域带来了巨大的机遇。在这里，我们描述了如何将这些抗生素选择系统与许多成熟的遗传方法相结合，以研究基因功能，从而提高线虫遗传工具包的时间和成本效益。