一种高度可重复的压迫性脊髓损伤小鼠模型。

A highly reproducible mouse model of compression spinal cord injury.

作者信息

Marques Suelen Adriani, de Almeida Fernanda Martins, Mostacada Klauss, Martinez Ana Maria Blanco

机构信息

Laboratório de Regeneração Neural e Função, Departamento de Neurobiologia, Fluminense Federal University, Niterói, RJ, Brazil.

出版信息

Methods Mol Biol. 2014;1162:149-56. doi: 10.1007/978-1-4939-0777-9_12.

DOI:10.1007/978-1-4939-0777-9_12

PMID:24838965

Abstract

Experimental spinal cord injury (SCI) can maintain the continuity of the spinal cord, as in the contusion (e.g., weight-fall) or compression models, or not, when there is a partial or a complete transection. The majority of acute human SCI is not followed by complete transection, but there is a combination of contusion, compression, and possibly partial transection. The method described here is a compressive mouse model that presents a combination of contusion and compression components and has many facilities in its execution. This lesion was established by our group and represents a simple, reliable, and inexpensive clip compression model with functional and morphological reproducibility. In this chapter we describe, step by step, the protocol of this experimental SCI.

摘要

实验性脊髓损伤（SCI）可以保持脊髓的连续性，如在挫伤（例如重物坠落）或压迫模型中，也可以在部分或完全横断时不保持连续性。大多数急性人类SCI并非完全横断，而是伴有挫伤、压迫，可能还有部分横断。这里描述的方法是一种压迫性小鼠模型，它呈现出挫伤和压迫成分的组合，并且在实施过程中有许多便利之处。这种损伤模型由我们团队建立，是一种简单、可靠且廉价的夹闭压迫模型，具有功能和形态学的可重复性。在本章中，我们将逐步描述这种实验性SCI的方案。