通过鉴定人精浆中的结合配体研究半乳糖凝集素-3在生殖道中的功能。
Investigation of galectin-3 function in the reproductive tract by identification of binding ligands in human seminal plasma.
作者信息
Kovak Matthew R, Saraswati Sarika, Schoen David J, Diekman Alan B
机构信息
Department of Biochemistry and Molecular Biology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
出版信息
Am J Reprod Immunol. 2014 Oct;72(4):403-12. doi: 10.1111/aji.12273. Epub 2014 May 23.
PROBLEM
Galectin-3 is a β-galactoside binding protein with immunomodulatory properties and exerts its extracellular functions via interactions with glycoconjugate ligands. Therefore, to elucidate the function of galectin-3, binding ligands in human seminal plasma were investigated.
METHOD OF STUDY
Galectin-3 binding proteins were isolated from seminal plasma by affinity chromatography, and candidate ligands were identified by MS/MS. Biochemical methods were used to characterize the ability of galectin-3 to bind its ligands.
RESULTS
Identified galectin-3 ligands included CD13, MUC6, PAP, PSA, and ZAG. 1D and 2D electrophoretic analysis of seminal plasma demonstrated that CD13, PAP, PSA, and ZAG immunoreactivity co-migrated with galectin-3-reactive protein bands and spots at expected molecular weights and pIs. Inhibition assays indicated that CD13, PSA, PAP, and ZAG interact with galectin-3 in a protein-carbohydrate manner.
CONCLUSION
The galectin-3 binding ligands identified in this study indicate multiple roles for galectin-3 in the reproductive and immunological functions of seminal plasma.
问题
半乳糖凝集素-3是一种具有免疫调节特性的β-半乳糖苷结合蛋白，通过与糖缀合物配体相互作用发挥其细胞外功能。因此，为阐明半乳糖凝集素-3的功能，对人精浆中的结合配体进行了研究。
研究方法
通过亲和色谱从精浆中分离半乳糖凝集素-3结合蛋白，并用串联质谱鉴定候选配体。采用生化方法表征半乳糖凝集素-3结合其配体的能力。
结果
鉴定出的半乳糖凝集素-3配体包括CD13、MUC6、前列腺酸性磷酸酶（PAP）、前列腺特异性抗原（PSA）和锌-α2-糖蛋白（ZAG）。精浆的一维和二维电泳分析表明，CD13、PAP、PSA和ZAG的免疫反应性与半乳糖凝集素-3反应性蛋白条带和斑点在预期分子量和等电点处共迁移。抑制试验表明，CD13、PSA、PAP和ZAG以蛋白质-碳水化合物的方式与半乳糖凝集素-3相互作用。
结论
本研究中鉴定出的半乳糖凝集素-3结合配体表明半乳糖凝集素-3在精浆的生殖和免疫功能中具有多种作用。
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