7-芳基-7-脱氮腺苷 2'-脱氧核苷三磷酸(dNTPs)：在竞争掺入中比 dATP 更适合 DNA 聚合酶的底物。
7-Aryl-7-deazaadenine 2'-deoxyribonucleoside triphosphates (dNTPs): better substrates for DNA polymerases than dATP in competitive incorporations.
机构信息
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, 16610 Prague 6 (Czech Republic) http://www.uochb.cas.cz/hocekgroup.
出版信息
Angew Chem Int Ed Engl. 2014 Jul 14;53(29):7552-5. doi: 10.1002/anie.201404742. Epub 2014 May 30.
A series of 7-substituted 7-deazaadenine and 5-substituted cytosine 2'-deoxyribonucleoside triphosphates (dNTPs) were tested for their competitive incorporations (in the presence of dATP and dCTP) into DNA by several DNA polymerases by using analysis based on cleavage by restriction endonucleases. 7-Aryl-7-deazaadenine dNTPs were more efficient substrates than dATP because of their higher affinity for the active site of the enzyme, as proved by kinetic measurements and calculations.
一系列 7-取代 7-脱氮腺嘌呤和 5-取代胞嘧啶 2'-脱氧核苷三磷酸(dNTPs)被测试其通过几种 DNA 聚合酶的竞争性掺入（在 dATP 和 dCTP 的存在下）进入 DNA，方法是基于限制内切酶切割的分析。7-芳基-7-脱氮腺嘌呤 dNTPs 比 dATP 更有效，因为它们与酶的活性位点的亲和力更高，这一点通过动力学测量和计算得到了证明。
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