Onoja Samuel Okwudili, Omeh Yusuf Ndukaku, Ezeja Maxwell Ikechukwu, Chukwu Martins Ndubuisi
Department of Veterinary Physiology, Pharmacology, Biochemistry and Animal Health and Production, College of Veterinary Medicine, Micheal Okpara University of Agriculture, PMB 7267, Umudike, Nigeria.
Department of Biochemistry, College of Natural Sciences, Micheal Okpara University of Agriculture, PMB 7267, Umudike, Nigeria.
J Trop Med. 2014;2014:159343. doi: 10.1155/2014/159343. Epub 2014 May 15.
Aframomum melegueta Schum (Zingiberaceae) is a perennial herb widely cultivated for its valuable seeds in the tropical region of Africa. The present study evaluated the antioxidant effects of methanolic seed extract of A. melegueta. The antioxidant effects were evaluated using in vitro, 2, 2-diphenylpicrylhydrazine photometric assay and in vivo serum catalase, superoxide dismutase and thiobarbituric acid reactive substance assay method. The extract (25-400 μ g/mL concentration) produced concentration dependent increase in antioxidant activity in 2, 2-diphenylpicrylhydrazine photometric assay. The extract (400 mg/kg) showed a significant (P < 0.05) increase in serum catalase and superoxide dismutase activity when compared with the control group. The extract (400 mg/kg) showed a significant (P < 0.05) decrease in the serum level of thiobarbituric acid reactive substance when compared with the control group. These findings suggest that the seed of A. melegueta has potent antioxidant activity which may be responsible for some of its reported pharmacological activities and can be used as antioxidant supplement.
非洲豆蔻（姜科）是一种多年生草本植物，因其珍贵的种子在非洲热带地区广泛种植。本研究评估了非洲豆蔻种子甲醇提取物的抗氧化作用。采用体外2,2-二苯基苦味酰基肼光度法以及体内血清过氧化氢酶、超氧化物歧化酶和硫代巴比妥酸反应性物质测定法评估抗氧化作用。在2,2-二苯基苦味酰基肼光度法中，提取物（浓度为25 - 400μg/mL）使抗氧化活性呈浓度依赖性增加。与对照组相比，提取物（400mg/kg）使血清过氧化氢酶和超氧化物歧化酶活性显著增加（P < 0.05）。与对照组相比，提取物（400mg/kg）使血清硫代巴比妥酸反应性物质水平显著降低（P < 0.05）。这些发现表明，非洲豆蔻种子具有强大的抗氧化活性，这可能是其一些已报道的药理活性的原因，并且可作为抗氧化补充剂使用。
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