Kaneko Keiko, Higuchi Chikahisa, Naka Norifumi, Yoshikawa Hideki
Department of Orthopedic Surgery, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Oncol Lett. 2014 Jul;8(1):111-116. doi: 10.3892/ol.2014.2138. Epub 2014 May 12.
Schwannoma is a benign peripheral nerve sheath tumor derived from Schwann cells. Bone scalloping, including spinal foramen enlargement, develops when the tumor is located adjacent to a bone and is a characteristic radiological feature. In the present study, to investigate the pathomechanism of bone resorption, the expression of noggin (a potent antagonist of bone morphogenetic protein) was analyzed in schwannoma tissues and compared with that observed in other soft tissue tumors. Quantitative polymerase chain reaction analysis revealed that the mRNA levels of noggin in schwannomas were significantly increased compared with the levels in other tumors. The gene product of noggin was only detected in a subset of schwannomas using immunohistochemistry and western blot analysis. Furthermore, the tissue extract from a noggin-producing schwannoma was found to inhibit osteoblastic differentiation in MC3T3 mouse osteoblastic cells in a dose-dependent manner. These findings indicate that bone scalloping in radiology may be induced by schwannoma-secreted noggin. In addition, noggin may have potential as a novel molecular and diagnostic marker for identifying certain types of schwannoma.
神经鞘瘤是一种起源于施万细胞的良性周围神经鞘膜肿瘤。当肿瘤位于骨骼附近时，会出现骨扇贝样改变，包括椎间孔扩大，这是一种典型的影像学特征。在本研究中，为了探究骨吸收的发病机制，分析了神经鞘瘤组织中骨形态发生蛋白的强效拮抗剂头蛋白（noggin）的表达，并与其他软组织肿瘤中的表达情况进行比较。定量聚合酶链反应分析显示，与其他肿瘤相比，神经鞘瘤中头蛋白的mRNA水平显著升高。使用免疫组织化学和蛋白质印迹分析仅在一部分神经鞘瘤中检测到头蛋白的基因产物。此外，发现来自产生头蛋白的神经鞘瘤的组织提取物以剂量依赖的方式抑制MC3T3小鼠成骨细胞的成骨分化。这些发现表明，影像学上的骨扇贝样改变可能是由神经鞘瘤分泌的头蛋白诱导的。此外，头蛋白可能具有作为鉴定某些类型神经鞘瘤的新型分子和诊断标志物 的潜力。
