Ma Chenchao, Nong Kate, Wu Bo, Dong Bo, Bai Yueqing, Zhu Hongda, Wang Weiwei, Huang Xinyu, Yuan Zhou, Ai Kaixing
Department of General Surgery, The Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, China.
J Exp Clin Cancer Res. 2014 Jun 25;33(1):54. doi: 10.1186/1756-9966-33-54.
microRNAs (miRNAs) are a class of small non-coding RNAs that play important roles in carcinogenesis. In the present study, we investigated the effect of miR-212 on pancreatic ductal adenocarcinoma (PDAC) and its target protein.
Quantitative real-time PCR(qRT-PCR) was performed to detect the expression of miR-212 in PDAC tissues and pancreatic cancer cell lines. miR-212 mimic, miR-212 inhibitor and negative control were transfected into pancreatic cancer cells and the effect of miR-212 up-regulation and down-regulation on the proliferation, migration and invasion of cells were investigated. Furthermore, the mRNA and protein levels of Patched-1(PTCH1) were measured. Meanwhile, luciferase assays were performed to validate PTCH1 as miR-212 target in PDAC.
miR-212 was up-regulated in PDAC tissues and cells.Using both gain-of function and loss-of function experiments, a pro-oncogenic function of miR-212 was demonstrated in PDAC. Moreover, up-regulated of PTCH1 could attenuate the effect induced by miR-212.
These data suggest that miR-212 could facilitate PDAC progression and metastasis through targeting PTCH1, implicating a novel mechanism for the progression of PDAC.
微小RNA（miRNA）是一类在致癌过程中发挥重要作用的小非编码RNA。在本研究中，我们研究了miR-212对胰腺导管腺癌（PDAC）及其靶蛋白的影响。
采用定量实时PCR（qRT-PCR）检测miR-212在PDAC组织和胰腺癌细胞系中的表达。将miR-212模拟物、miR-212抑制剂和阴性对照转染到胰腺癌细胞中，研究miR-212上调和下调对细胞增殖、迁移和侵袭的影响。此外，检测了Patched-1（PTCH1）的mRNA和蛋白水平。同时，进行荧光素酶报告基因实验以验证PTCH1是PDAC中miR-212的靶标。
miR-212在PDAC组织和细胞中上调。通过功能获得和功能缺失实验，均证明miR-212在PDAC中具有促癌功能。此外，PTCH1的上调可减弱miR-212诱导的效应。
这些数据表明，miR-212可通过靶向PTCH1促进PDAC的进展和转移，这为PDAC的进展提供了一种新机制。
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