褪黑素受体1B基因rs4753426单核苷酸多态性与妊娠期糖尿病的相关性

[Association between single nucleotide polymorphism of rs4753426 of melatonin receptor 1B gene and gestational diabetes mellitus].

作者信息

Zhan Ying, Liu Furong, Li Chao, Gao Qun, Liu Shiguo, Wang Yuping

机构信息

Department of Obstetrics, the Affiliated Hospital of Qingdao University, Qingdao 266003, China. Email:

Department of Obstetrics, the Affiliated Hospital of Qingdao University, Qingdao 266003, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2014 Apr;49(4):276-80.

PMID:24969335

Abstract

OBJECTIVE

To investigate the genotypic and allele frequency differences of melatonin receptor 1B (MTNR1B)-rs4753426 between gestational diabetes mellitus (GDM) pregnancies and normal pregnancies, and to explore the association between single nucleotide polymorphism (SNP) of rs4753426 and gestational diabetes mellitus.

METHODS

Totally 93 GDM pregnancies and 165 normal pregnancies were recruited from the Affiliated Hospital of Qingdao University. The age, gestational weeks, height, early pregnant weight, and the levels of fasting plasma glucose (FPG), fasting insulin (FIN) were determined in every participants. By using PCR and DNA sequencing, we detected the distribution of the rs4753426 genotypes and alleles in all individuals. The homeostasis model assessment-insulin resistance (HOMA-IR) and the homeostasis model assessment-β cell function (HOMA-β) were calculated. The allele and genotype frequencies and the FPG, FIN, body mass index (BMI), HOMA-IR, HOMA-β levels between GDM group and control group were compared.

RESULTS

(1)The genotype frequencies in the GDM group and the control group of rs4753426-CC, CT, TT were 72.0% (67/93), 21.5% (20/93), 6.5% (6/93), and 53.9% (89/165), 40.0% (66/165), 6.1% (10/165) respectively. The allele frequencies in the GDM group and the control group of T and C were 17.2% (32/186), 82.8% (154/186) and 26.1% (86/330), 73.9% (244/330) respectively. There were statistical differences in genotype frequencies and allele frequencies between two groups (all P < 0.05). (2)The levels of FPG, FIN and HOMA-IR in the GDM group were obviously higher than those in the control group (P < 0.05). The level of HOMA-β was lower in the GDM group than that of the control group (P < 0.05).(3)The FPG of CC and CT genotypes was higher than that of TT genotype in the GDM group (P < 0.05), while the level of HOMA-β was lower than that of TT genotype (P < 0.05).

CONCLUSIONS

The MTNR1B-rs4753426 SNP is associated with the pathogenesis of GDM, and rs4753426 is the predisposing locus of GDM. The C-allele is the susceptibility allele of GDM.

摘要

目的

探讨妊娠期糖尿病（GDM）孕妇与正常孕妇之间褪黑素受体1B（MTNR1B）-rs4753426的基因型和等位基因频率差异，并探讨rs4753426单核苷酸多态性（SNP）与妊娠期糖尿病的相关性。

方法

从青岛大学附属医院招募93例GDM孕妇和165例正常孕妇。测定每位参与者的年龄、孕周、身高、早孕体重以及空腹血糖（FPG）、空腹胰岛素（FIN）水平。采用聚合酶链反应（PCR）和DNA测序技术，检测所有个体中rs4753426基因型和等位基因的分布。计算稳态模型评估胰岛素抵抗（HOMA-IR）和稳态模型评估β细胞功能（HOMA-β）。比较GDM组和对照组的等位基因和基因型频率以及FPG、FIN、体重指数（BMI）、HOMA-IR、HOMA-β水平。

结果

（1）rs4753426-CC、CT、TT基因型在GDM组和对照组中的频率分别为72.0%（67/93）、21.5%（20/93）、6.5%（6/93）和53.9%（89/165）、40.0%（66/165）、6.1%（10/165）。GDM组和对照组中T和C等位基因频率分别为17.2%（32/186）、82.8%（154/186）和26.1%（86/330）、73.9%（244/330）。两组间基因型频率和等位基因频率存在统计学差异（均P<0.05）。（2）GDM组的FPG水平、FIN水平和HOMA-IR明显高于对照组（P<0.05）。GDM组的HOMA-β水平低于对照组（P<0.05）。（3）GDM组中CC和CT基因型的FPG高于TT基因型（P<0.05），而HOMA-β水平低于TT基因型（P<0.05）。