前列腺癌骨转移的荧光引导手术

Fluorescence-guided surgery of prostate cancer bone metastasis.

作者信息

Miwa Shinji, Matsumoto Yasunori, Hiroshima Yukihiko, Yano Shuya, Uehara Fuminari, Yamamoto Mako, Zhang Yong, Kimura Hiroaki, Hayashi Katsuhiro, Yamamoto Norio, Bouvet Michael, Sugimoto Naotoshi, Tsuchiya Hiroyuki, Hoffman Robert M

机构信息

AntiCancer, Inc, San Diego, California; Department of Surgery, University of California, San Diego, San Diego, California; Department of Orthopedic Surgery, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Ishikawa, Japan.

Department of Surgery, University of California, San Diego, San Diego, California.

出版信息

J Surg Res. 2014 Nov;192(1):124-33. doi: 10.1016/j.jss.2014.05.049. Epub 2014 May 23.

DOI:10.1016/j.jss.2014.05.049

PMID:24972740

Abstract

BACKGROUND

The aim of this study is to investigate the effectiveness of fluorescence-guided surgery (FGS) of prostate cancer experimental skeletal metastasis.

MATERIALS AND METHODS

Green fluorescent protein-expressing PC-3 human prostate cancer cells (PC-3-green fluorescent protein) were injected into the intramedullary cavity of the tibia in 32 nude mice. After 2 wk, 16 of the mice underwent FGS; the other 16 mice underwent bright-light surgery (BLS). Half of BLS and FGS mice (8 mice in each group) received zoledronic acid (ZOL). Weekly fluorescence imaging of the mice was performed. Six weeks after surgery, metastases to lung and inguinal lymph node were evaluated by fluorescence imaging.

RESULTS

The percentage of residual tumor after BLS and FGS was 9.9 ± 2.2% and 0.9 ± 0.3%, respectively (P < 0.001). FGS reduced recurrent cancer growth compared with BLS (P < 0.005). Although FGS alone had no significant effect on inguinal lymph node metastases, lung metastasis or disease-free survival (DFS), ZOL in combination with FGS significantly increased DFS (P = 0.01) in comparison with the combination of BLS and ZOL. ZOL reduced lymph node metastases (P = 0.033) but not lung metastasis.

CONCLUSIONS

FGS significantly reduced recurrence of experimental prostate cancer bone metastasis compared with BLS. The combination of FGS and ZOL increased DFS over BLS and ZOL. ZOL inhibited lymph node metastasis but not lung metastasis.

摘要

背景

本研究旨在探讨荧光引导手术（FGS）对前列腺癌实验性骨转移的有效性。

材料与方法

将表达绿色荧光蛋白的PC-3人前列腺癌细胞（PC-3-绿色荧光蛋白）注射到32只裸鼠的胫骨骨髓腔内。2周后，16只小鼠接受FGS；另外16只小鼠接受强光手术（BLS）。BLS组和FGS组各有一半小鼠（每组8只）接受唑来膦酸（ZOL）治疗。每周对小鼠进行荧光成像。术后6周，通过荧光成像评估肺和腹股沟淋巴结转移情况。

结果

BLS和FGS术后的残余肿瘤百分比分别为9.9±2.2%和0.9±0.3%（P<0.001）。与BLS相比，FGS减少了复发性癌症生长（P<0.005）。尽管单独的FGS对腹股沟淋巴结转移、肺转移或无病生存期（DFS）没有显著影响，但与BLS联合ZOL相比，ZOL联合FGS显著提高了DFS（P=0.01）。ZOL减少了淋巴结转移（P=0.033），但对肺转移没有影响。