重复使用稳定磁性絮凝剂高效收获油脂性小球藻
Repeated use of stable magnetic flocculant for efficient harvest of oleaginous Chlorella sp.
机构信息
Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea.
Biomass and Waste Energy Laboratory, Korea Institute of Energy Research (KIER), 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea; Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, Republic of Korea.
出版信息
Bioresour Technol. 2014 Sep;167:284-90. doi: 10.1016/j.biortech.2014.06.055. Epub 2014 Jun 20.
In the present study, a simple magnetic-particle recycling strategy was developed for harvest of the oleaginous microalga Chlorella sp. KR-1. The method entails the flocculation of microalgal cells and bare-Fe3O4 magnetic particles (bMP) by electrostatic attraction and the subsequent recovery of the bMP from the harvested flocs by electrostatic repulsion below and above the isoelectric points (IEP), respectively. For 10 recycles, the bMP showed 94-99% and 90-97% harvest and recovery efficiencies, respectively. Furthermore, neither the use of bMP nor pH adjustment showed any adverse effect on the microalgal cell growth or the co-existing bacterial species, as confirmed from the subsequent medium-recycling test and denaturing gradient gel electrophoresis (DGGE) analysis.
在本研究中，开发了一种简单的磁性粒子回收策略，用于收获油脂微藻小球藻 KR-1。该方法涉及通过静电吸引絮凝微藻细胞和 bare-Fe3O4 磁性粒子（bMP），并分别在低于和高于等电点（IEP）时通过静电排斥从收获的絮体中回收 bMP。在 10 次循环中，bMP 的收获和回收率分别达到 94-99%和 90-97%。此外，正如随后的培养基再循环试验和变性梯度凝胶电泳（DGGE）分析所证实的，bMP 的使用或 pH 值调整均未对微藻细胞生长或共存的细菌种类产生任何不利影响。
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