用于酶固定化的聚磷腈水凝胶的制备
Preparation of polyphosphazene hydrogels for enzyme immobilization.
作者信息
Qian Yue-Cheng, Chen Peng-Cheng, He Gui-Jin, Huang Xiao-Jun, Xu Zhi-Kang
机构信息
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
出版信息
Molecules. 2014 Jul 8;19(7):9850-63. doi: 10.3390/molecules19079850.
We report on the synthesis and application of a new hydrogel based on a methacrylate substituted polyphosphazene. Through ring-opening polymerization and nucleophilic substitution, poly[bis(methacrylate)phosphazene] (PBMAP) was successfully synthesized from hexachlorocyclotriphosphazene. By adding PBMAP to methacrylic acid solution and then treating with UV light, we could obtain a cross-linked polyphosphazene network, which showed an ultra-high absorbency for distilled water. Lipase from Candida rugosa was used as the model lipase for entrapment immobilization in the hydrogel. The influence of methacrylic acid concentration on immobilization efficiency was studied. Results showed that enzyme loading reached a maximum of 24.02 mg/g with an activity retention of 67.25% when the methacrylic acid concentration was 20% (w/w).
我们报道了一种基于甲基丙烯酸酯取代聚磷腈的新型水凝胶的合成及应用。通过开环聚合和亲核取代反应，由六氯环三磷腈成功合成了聚[双（甲基丙烯酸酯）磷腈]（PBMAP）。将PBMAP加入甲基丙烯酸溶液中，然后用紫外光处理，可得到交联聚磷腈网络，该网络对蒸馏水显示出超高吸水性。使用皱褶假丝酵母脂肪酶作为模型脂肪酶，将其包埋固定在水凝胶中。研究了甲基丙烯酸浓度对固定化效率的影响。结果表明，当甲基丙烯酸浓度为20%（w/w）时，酶负载量最高可达24.02 mg/g，活性保留率为67.25%。
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