沙特女性中对氧磷酶1基因谷氨酰胺192与精氨酸多态性和妊娠期糖尿病之间的关系
Relationship between the paraoxonase 1 gene glutamine 192 to arginine polymorphism and gestational diabetes mellitus in Saudi women.
作者信息
Al-Hakeem Malak Mohammed, Abotalib Zeinab, Alharbi Khalid Khalaf, Khan Imran Ali
机构信息
Department of Obstetrics and Gynecology, King Khalid University Hospital, College of Medicine, King Saud University, P.O. Box 60826, Riyadh 11555, Saudi Arabia.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
出版信息
Clin Biochem. 2014 Oct;47(15):122-5. doi: 10.1016/j.clinbiochem.2014.07.005. Epub 2014 Jul 15.
OBJECTIVES
Gestational diabetes mellitus (GDM) is recognized as an imbalance between insulin resistance and insulin secretion, leading to maternal hyperglycemia. Previous studies in a Saudi population indicated a high frequency of Paraoxonase 1 glutamine 192 to arginine (PON1 Q192R) polymorphism, suggesting this polymorphism as an additional risk factor. The present study was designed to explore the possible association between the PON1 Q192R polymorphism and GDM in a Saudi population.
METHODS
This case-control study was carried out in 500 pregnant women, including 200 GDM cases and 300 non-GDM women. Genotyping for PON1 Q192R (rs662) variants was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
RESULTS
The results of the present study indicates that Q192R polymorphism was significantly associated with GDM in a Saudi population with the minor allele frequency (MAF) (p=0.0007). Q192R genotypes and alleles showed a strong association with GDM (p=0.009 and p=0.0007, respectively).
CONCLUSION
In conclusion, these findings suggest that the PON1 Q192R polymorphism has high MAF in GDM in the studied Saudi population.
目的
妊娠期糖尿病（GDM）被认为是胰岛素抵抗与胰岛素分泌之间的失衡，导致母体高血糖。先前在沙特人群中的研究表明，对氧磷酶1谷氨酰胺192位点突变为精氨酸（PON1 Q192R）的多态性频率较高，提示这种多态性是一个额外的风险因素。本研究旨在探讨沙特人群中PON1 Q192R多态性与GDM之间可能存在的关联。
方法
本病例对照研究纳入500名孕妇，其中包括200例GDM患者和300例非GDM女性。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对PON1 Q192R（rs662）变体进行基因分型。
结果
本研究结果表明，在沙特人群中，Q192R多态性与GDM显著相关，次要等位基因频率（MAF）（p = 0.0007）。Q192R基因型和等位基因与GDM均表现出强相关性（分别为p = 0.009和p = 0.0007）。
结论
总之，这些发现表明，在所研究的沙特人群中，GDM患者中PON1 Q192R多态性的MAF较高。
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