结核分枝杆菌酚糖脂抗原（PGL-Tb 1）在结核病血清学诊断中的评估：与结核菌素纯蛋白衍生物抗原的比较

Evaluation of a phenolglycolipid antigen (PGL-Tb 1) from M. tuberculosis in the serodiagnosis of tuberculosis: comparison with PPD antigen.

作者信息

Martín Casabona N, Gonzalez Fuente T, Arcalis Arce L, Otal Entraigas J, Vidal Pla R

机构信息

Laboratory of Microbiology and Parasitology, Ciutat Sanitària Vall d'Hebron, Universitat Autònoma de Barcelona, Spain.

出版信息

Acta Leprol. 1989;7 Suppl 1:89-93.

PMID:2504014

Abstract

Sera from 38 tuberculous patients and 62 healthy controls (31 PPD skin test positive and 31 negative) were assayed, by enzyme-linked immunosorbent assay (ELISA), to test the activity of IgG and IgM antibodies against purified protein derivative (PPD) antigen and a phenolglycolipid antigen (PLG-Tb 1) isolated and purified from Mycobacterium tuberculosis strain Canetti. Using PPD antigen, the sensitivity and specificity were respectively, 50 and 93.5% for IgG and 71.1 and 59.7% for IgM antibody activity. Against PGL-Tb 1 antigen, IgG had a sensitivity of 94.7% and the specificity was 96.8%, for IgM antibody they were 65.8% and 75.8% respectively. The ELISA using PGL-Tb 1 antigen could be a useful way to develop a rapid technique to aid in the diagnosis of tuberculosis.

摘要

采用酶联免疫吸附测定法（ELISA）检测了38例结核病患者及62名健康对照者（31名PPD皮肤试验阳性，31名阴性）的血清，以检测针对纯化蛋白衍生物（PPD）抗原以及从结核分枝杆菌卡内蒂菌株中分离纯化出的酚糖脂抗原（PLG-Tb 1）的IgG和IgM抗体活性。使用PPD抗原时，IgG抗体活性的敏感性和特异性分别为50%和93.5%，IgM抗体活性的敏感性和特异性分别为71.1%和59.7%。针对PGL-Tb 1抗原，IgG的敏感性为94.7%，特异性为96.8%，IgM抗体的敏感性和特异性分别为65.8%和75.8%。使用PGL-Tb 1抗原的ELISA法可能是开发一种有助于结核病诊断的快速技术的有用方法。