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沼泽鞘氨醇杆菌新种,分离自湿地土壤。

Sphingobacterium paludis sp. nov., isolated from wetland soil.

机构信息

Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, PR China.

出版信息

Int J Syst Evol Microbiol. 2014 Oct;64(Pt 10):3453-3458. doi: 10.1099/ijs.0.064915-0. Epub 2014 Jul 21.

Abstract

A novel Gram-stain-negative bacteria, designated S37(T), was isolated from soil of the Xixi wetland, Zhejiang province, China. Cells of strain S37(T) were aerobic, non-motile rods. Growth occurred at 10-37 °C (optimum, 25 °C), pH 5.0-9.7 (optimum, pH 7.5) and with 0-6% (w/v) NaCl (optimum, 0.5%). Based on 16S rRNA gene sequence analysis, strain S37(T) was found to be a member of the genus Sphingobacterium and shared highest similarity with Sphingobacterium composti 4M24(T) (95.78%). The major fatty acids were summed feature 3 (iso-C15:0 2-OH and/or C16:1ω7c), iso-C15:0 and iso-C17:0 3-OH, and the DNA G+C content was 43.8 mol%. The predominant respiratory quinone was MK-7. Based on its phenotypic and chemotaxonomic characteristics and phylogenetic data, strain S37(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium paludis sp. nov. (type strain S37(T) = CGMCC 1.12801(T) = NBRC 110386(T)) is proposed.

摘要

一株新型革兰氏阴性菌,命名为 S37(T),从中国浙江省西溪湿地的土壤中分离得到。S37(T) 菌株的细胞为需氧、非运动棒状。生长的温度范围为 10-37°C(最佳温度为 25°C),pH 值范围为 5.0-9.7(最佳 pH 值为 7.5),能在 0-6%(w/v)NaCl 中生长(最佳为 0.5%)。根据 16S rRNA 基因序列分析,S37(T) 菌株属于鞘氨醇单胞菌属,与 Sphingobacterium composti 4M24(T)(95.78%)最为相似。主要脂肪酸为 sum feature 3(iso-C15:0 2-OH 和/或 C16:1ω7c)、iso-C15:0 和 iso-C17:0 3-OH,DNA G+C 含量为 43.8 mol%。主要的呼吸醌为 MK-7。基于其表型和化学分类特征以及系统发育数据,S37(T) 菌株代表了鞘氨醇单胞菌属的一个新型物种,将其命名为 Sphingobacterium paludis sp. nov.(模式菌株 S37(T) = CGMCC 1.12801(T) = NBRC 110386(T))。

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