Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Department of Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, PR China.
Int J Syst Evol Microbiol. 2014 Oct;64(Pt 10):3453-3458. doi: 10.1099/ijs.0.064915-0. Epub 2014 Jul 21.
A novel Gram-stain-negative bacteria, designated S37(T), was isolated from soil of the Xixi wetland, Zhejiang province, China. Cells of strain S37(T) were aerobic, non-motile rods. Growth occurred at 10-37 °C (optimum, 25 °C), pH 5.0-9.7 (optimum, pH 7.5) and with 0-6% (w/v) NaCl (optimum, 0.5%). Based on 16S rRNA gene sequence analysis, strain S37(T) was found to be a member of the genus Sphingobacterium and shared highest similarity with Sphingobacterium composti 4M24(T) (95.78%). The major fatty acids were summed feature 3 (iso-C15:0 2-OH and/or C16:1ω7c), iso-C15:0 and iso-C17:0 3-OH, and the DNA G+C content was 43.8 mol%. The predominant respiratory quinone was MK-7. Based on its phenotypic and chemotaxonomic characteristics and phylogenetic data, strain S37(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium paludis sp. nov. (type strain S37(T) = CGMCC 1.12801(T) = NBRC 110386(T)) is proposed.
一株新型革兰氏阴性菌,命名为 S37(T),从中国浙江省西溪湿地的土壤中分离得到。S37(T) 菌株的细胞为需氧、非运动棒状。生长的温度范围为 10-37°C(最佳温度为 25°C),pH 值范围为 5.0-9.7(最佳 pH 值为 7.5),能在 0-6%(w/v)NaCl 中生长(最佳为 0.5%)。根据 16S rRNA 基因序列分析,S37(T) 菌株属于鞘氨醇单胞菌属,与 Sphingobacterium composti 4M24(T)(95.78%)最为相似。主要脂肪酸为 sum feature 3(iso-C15:0 2-OH 和/或 C16:1ω7c)、iso-C15:0 和 iso-C17:0 3-OH,DNA G+C 含量为 43.8 mol%。主要的呼吸醌为 MK-7。基于其表型和化学分类特征以及系统发育数据,S37(T) 菌株代表了鞘氨醇单胞菌属的一个新型物种,将其命名为 Sphingobacterium paludis sp. nov.(模式菌株 S37(T) = CGMCC 1.12801(T) = NBRC 110386(T))。