用于牙髓药物递送载体的光固化水凝胶的初步研究。
Preliminary study of light-cured hydrogel for endodontic drug delivery vehicle.
作者信息
Komabayashi Takashi, Wadajkar Aniket, Santimano Sonia, Ahn Chul, Zhu Qiang, Opperman Lynn A, Bellinger Larry L, Yang Jian, Nguyen Kytai T
机构信息
Department of Endodontics, West Virginia University School of Dentistry, Morgantown, WV, USA.
Department of Bioengineering, University of Texas, Arlington, TX, USA.
出版信息
J Investig Clin Dent. 2016 Feb;7(1):87-92. doi: 10.1111/jicd.12118. Epub 2014 Jul 22.
AIM
Direct pulp capping is the treatment of an exposed vital pulp with a dental material to facilitate the formation of reparative dentin and maintenance of vital pulp. A bioengineered drug delivery vehicle has the potential to increase the success rate of pulp capping. The aim of this study was to develop an injectable and light-curing drug delivery vehicle for endodontic treatment including direct pulp capping.
METHODS
Polyethylene glycol-maleate-citrate (PEGMC) hydrogel was synthesized as a drug delivery vehicle that is composed of PEGMC (45% w/v), acrylic acid (AA) (5% w/v), 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AAPH) (0.1% w/v), and deionized water. The association between prehydrogel-solution volume and visible light-curing was examined. The cytotoxicity of the hydrogel was tested using L929 cells in a cell culture system. Ca(2+) release from the hydrogel was determined using calcium hydroxide as the incorporated medicine.
RESULTS
The results showed that the light-curing time for hydrogel is comparable to composite resin. The hydrogel had cell toxicity similar to adhesive systems. Moreover, controlled Ca(2+) release was obtained from the calcium hydroxide incorporated hydrogel.
CONCLUSIONS
The data suggest that hydrogel should be explored further as a promising drug delivery vehicle for vital pulp therapy and regenerative endodontics.
目的
直接盖髓术是用牙科材料处理暴露的活髓，以促进修复性牙本质的形成并维持牙髓活力。一种生物工程药物递送载体有可能提高盖髓术的成功率。本研究的目的是开发一种用于牙髓治疗（包括直接盖髓术）的可注射且可光固化的药物递送载体。
方法
合成聚乙二醇 - 马来酸酯 - 柠檬酸盐（PEGMC）水凝胶作为药物递送载体，其由PEGMC（45% w/v）、丙烯酸（AA）（5% w/v）、2,2'-偶氮二异丁脒二盐酸盐（AAPH）（0.1% w/v）和去离子水组成。研究了预水凝胶溶液体积与可见光固化之间的关系。在细胞培养系统中使用L929细胞测试水凝胶的细胞毒性。以氢氧化钙作为掺入药物，测定水凝胶中Ca(2+)的释放情况。
结果
结果表明，水凝胶的光固化时间与复合树脂相当。该水凝胶具有与粘结系统相似的细胞毒性。此外，从掺入氢氧化钙的水凝胶中实现了Ca(2+)的可控释放。
结论
数据表明，水凝胶作为一种有前景的用于活髓治疗和再生牙髓病学的药物递送载体，应进一步探索。
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