一种快速构建假单胞菌 HF-1 生物强化系统的新方法：通过 pH 调节加速酰化高丝氨酸内酯的分泌。
A new method for rapid construction of a Pseudomonas sp. HF-1 bioaugmented system: accelerating acylated homoserine lactones secretion by pH regulation.
机构信息
School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China.
School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.
出版信息
Bioresour Technol. 2014 Oct;169:229-235. doi: 10.1016/j.biortech.2014.06.098. Epub 2014 Jul 3.
Pseudomonas sp. HF-1 bioaugmented systems were operated to treat tobacco wastewater under pH 5.5 for three cycles and pH 8.0 for the rest, which was suitable for HF-1 biofilm formation. The results showed that, under pH control, the contents of 3-oxo-C6-HSL, C6-HSL and 3-oxo-C8-HSL were significantly higher than HF-1 thresholds for biofilm formation. Compared with non-pH controlled reactors, HF-1 showed greater colonization in pH controlled reactors, primarily owing to the high extracellular polymeric substances secretion induced by quorum sensing. Accordingly, high indigenous community activity and granular sludge were observed. Sludge granulation occurred from the seventh cycle, and the average diameter was greater than 400 μm. These systems were also highly efficient with nearly 100% nicotine degradation and 60% total organic carbon removal. Overall, the results indicate that pH regulation is a new and feasible method for acceleration of releasing of auto-inducers, which is beneficial to construction of HF-1 bioaugmented systems.
HF-1 假单胞菌生物增强系统在 pH 值为 5.5 下运行三个周期，然后在其余时间内调节 pH 值至 8.0，以适应 HF-1 生物膜的形成。结果表明，在 pH 值控制下，3-氧代-C6-HSL、C6-HSL 和 3-氧代-C8-HSL 的含量显著高于 HF-1 生物膜形成的阈值。与非 pH 值控制的反应器相比，HF-1 在 pH 值控制的反应器中表现出更大的定殖能力，主要是由于群体感应诱导的胞外聚合物物质的大量分泌。因此，观察到高土著群落活性和颗粒污泥。从第七个周期开始出现污泥颗粒化，平均直径大于 400μm。这些系统的尼古丁去除率接近 100%，总有机碳去除率达到 60%，效率非常高。总的来说，结果表明 pH 值调节是一种新的、可行的方法，可以加速自动诱导物的释放，有利于 HF-1 生物增强系统的构建。
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