肝部分切除后大鼠 EGF 基因启动子中的 DNA 甲基化动态。

DNA methylation dynamics in the rat EGF gene promoter after partial hepatectomy.

机构信息

Key Laboratory for Cell Differentiation Regulation , Xinxiang , China . ; College of Life Science , Henan Normal University , Xinxiang , China .

出版信息

Genet Mol Biol. 2014 Jun;37(2):439-43. doi: 10.1590/s1415-47572014000300017.

DOI:10.1590/s1415-47572014000300017

PMID:25071410

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4094617/

Abstract

Epidermal growth factor (EGF), a multifunctional growth factor, is a regulator in a wide variety of physiological processes. EGF plays an important role in the regulation of liver regeneration. This study was aimed at investigating the methylation level of EGF gene throughout liver regeneration. DNA of liver tissue from control rats and partial hepatectomy (PH) rats at 10 time points was extracted and a 354 bp fragment including 10 CpG sites from the transcription start was amplified after DNA was modified by sodium bisulfate. The result of sequencing suggested that methylation ratio of four CpG sites was found to be significantly changed when PH group was compared to control group, in particular two of them were extremely striking. mRNA expression of EGF was down-regulated in total during liver regeneration. We think that the rat EGF promoter region is regulated by variation in DNA methylation during liver regeneration.

摘要

表皮生长因子（EGF）是一种多功能生长因子，是多种生理过程的调节剂。EGF 在肝脏再生的调节中发挥着重要作用。本研究旨在研究 EGF 基因在整个肝脏再生过程中的甲基化水平。从对照组大鼠和部分肝切除（PH）大鼠的肝脏组织中提取 DNA，并在 DNA 经亚硫酸氢钠修饰后扩增包括转录起始点 10 个 CpG 位点在内的 354bp 片段。测序结果表明，与对照组相比，PH 组有 4 个 CpG 位点的甲基化比率发现有明显变化，其中两个位点变化非常显著。在整个肝脏再生过程中 EGF 的 mRNA 表达均下调。我们认为，大鼠 EGF 启动子区域在肝脏再生过程中受 DNA 甲基化变化的调节。