Recombinant protein expression in microbial systems.
作者信息
Rosano Germán L, Ceccarelli Eduardo A
机构信息
Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario Rosario, Argentina.
出版信息
Front Microbiol. 2014 Jul 8;5:341. doi: 10.3389/fmicb.2014.00341. eCollection 2014.
Abstract
摘要
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4
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Front Microbiol. 2014 Feb 19;5:63. doi: 10.3389/fmicb.2014.00063. eCollection 2014.
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Front Microbiol. 2014 Feb 17;5:60. doi: 10.3389/fmicb.2014.00060. eCollection 2014.
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