曼氏血吸虫成虫抗原和可溶性虫卵抗原在免疫对流电泳（CIEP）、间接血凝试验（IHA）及酶联免疫吸附测定（ELISA）用于血吸虫病血清学诊断中的应用。

The application of S. mansoni adult and soluble egg antigens for the serodiagnosis of schistosomiasis by CIEP, IHA and ELISA.

作者信息

Khalil H M, Makled M K, Elmissiry A G, Khalil N M, Sonbol S E

出版信息

J Egypt Soc Parasitol. 1989 Dec;19(2 Suppl):827-43.

Abstract

The sero-reactivity of 82 schistosomiasis infected patients were studied by the use of the CIEP, IHA and ELISA tests using S. mansoni adult worm antigen (AWA) and soluble egg antigen (SEA). CIEP with (AWA) showed a progressive sensitivity decreased with advance of S. mansoni infection, and increased in sensitivity by (SEA). The test was more sensitive in the early cases of S. haematobium infection by (AWA), while by (SEA) it was more sensitive in the late cases. There was no difference in sensitivity of the test with either antigens in mixed bilharzial infection. IHAT with (AWA) showed high sensitivity in the different stages of S. mansoni infection, but sensitivity decreased with the progress of clinical status of S. mansoni if (SEA) was utilized. The test with (AWA) was slightly more sensitive in the early cases of S. haematobium than the late cases, while the same high sensitivity was given by the test with (SEA) in both groups. Also IHAT with (AWA) was more sensitive for the diagnosis of mixed bilharzial infection than with (SEA). ELISA test with (SEA) showed a progressive increase in sensitivity with the advance of S. mansoni. Also the test with (SEA) was more sensitive than with (AWA); and for the diagnosis of cases of mixed bilharzial infection, while S. haematobium infected cases showed a lower sensitivity than S. mansoni or mixed bilharzial infection cases, if the ELISA test is performed with either antigens.

摘要

使用曼氏血吸虫成虫抗原（AWA）和可溶性虫卵抗原（SEA），通过对流免疫电泳（CIEP）、间接血凝试验（IHA）和酶联免疫吸附测定（ELISA）检测，对82例血吸虫病感染患者的血清反应性进行了研究。用（AWA）进行的CIEP显示，随着曼氏血吸虫感染的进展，敏感性逐渐降低，而用（SEA）时敏感性增加。在用（AWA）检测早期埃及血吸虫感染病例时该试验更敏感，而在用（SEA）检测时晚期病例更敏感。在混合血吸虫感染中，使用任何一种抗原进行检测的敏感性均无差异。用（AWA）进行的间接血凝试验（IHAT）在曼氏血吸虫感染的不同阶段均显示出高敏感性，但如果使用（SEA），则随着曼氏血吸虫临床病情的进展敏感性降低。在用（AWA）检测时，埃及血吸虫早期病例的试验敏感性略高于晚期病例，而在用（SEA）检测时两组的敏感性均较高。此外，用（AWA）进行的IHAT对混合血吸虫感染的诊断比用（SEA）更敏感。用（SEA）进行的ELISA试验显示，随着曼氏血吸虫感染的进展，敏感性逐渐增加。而且用（SEA）进行的试验比用（AWA）更敏感；对于混合血吸虫感染病例的诊断，而埃及血吸虫感染病例在用任何一种抗原进行ELISA检测时，其敏感性均低于曼氏血吸虫或混合血吸虫感染病例。