The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology.
National Information Communications Technology Australia Victoria Research Laboratory, Department of Electrical and Electronic Engineering, School of Engineering.
Blood. 2014 Oct 23;124(17):2725-9. doi: 10.1182/blood-2014-02-559468. Epub 2014 Jul 25.
In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.
在这项研究中，我们验证了这样一个假设，即胚胎卵黄囊（YS）中的造血祖细胞/集落形成细胞具有巨核细胞潜能，它们在体内分化为第一批血小板形成细胞。我们证明，从胚胎第 8.5 天（E）开始，所有巨核细胞（MK）集落形成细胞都属于常规造血祖细胞（HPC）区室。尽管这些细胞确实能够产生多倍体 MK，但它们不是第一批血小板形成细胞的来源。我们表明，血小板前体细胞的形成首先发生在一个独特的、以前未被识别的二倍体血小板形成细胞谱系中，该谱系与 HPC 平行发育于 YS 中，但在 E8.5 Runx1 缺失胚胎中可以被指定，尽管缺乏祖细胞谱系。
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