[使用单克隆抗体对两种高纯度凝血因子VIII浓缩剂（Hemofil M和Monoclate）中凝血因子VIII/血管性血友病因子特性的研究]

[Properties of factor VIII/von Willebrand factor in two highly-purified factor VIII concentrates, Hemofil M and Monoclate, using monoclonal antibodies].

作者信息

Tanaka I, Yoshioka A, Shima M, Fujiwara T, Terada S, Nakai H, Miyata S, Sawamoto Y, Kamisue S, Fukui H

出版信息

Rinsho Ketsueki. 1989 Jul;30(7):951-7.

Abstract

Properties of F. VIII/vWF in highly-purified factor VIII concentrates were examined using monoclonal antibodies. The F. VIII: C levels obtained with the chromogenic assay agreed with those obtained with the one-stage clotting method. The ratio between F. VIII: Ag and F. VII: C in Hemofil M and Monoclate was 1.09 and 1.34, respectively. The F. VIII: Ag levels assayed by monoclonal ELISAs were the same as those assayed by polyclonal ELISA, except that those assayed by C 5-ELISA tended to be higher. The ratio between F. VIII: Ag and vWF: Ag in Hemofil M and Monoclate was 105 and 45, respectively. Both concentrates lacked the large multimers of vWF and showed the intensification of the satellite bands. SDS-PAGE patterns showed almost no contamination. Immunoblot analysis revealed that F. VIII in both concentrates could react with 6 kinds of monoclonal antibodies to F. VIII. These results suggest that the fundamental structure of F. VIII molecule for coagulant activity in both concentrates are preserved.

摘要

使用单克隆抗体检测高纯度凝血因子 VIII 浓缩物中 F. VIII/vWF 的特性。发色底物法测得的 F. VIII: C 水平与一期凝血法测得的结果一致。Hemofil M 和 Monoclate 中 F. VIII: Ag 与 F. VII: C 的比值分别为 1.09 和 1.34。单克隆 ELISA 法检测的 F. VIII: Ag 水平与多克隆 ELISA 法检测的结果相同，只是 C 5-ELISA 法检测的结果往往偏高。Hemofil M 和 Monoclate 中 F. VIII: Ag 与 vWF: Ag 的比值分别为 105 和 45。两种浓缩物均缺乏 vWF 的大分子量多聚体，并显示卫星带增强。SDS-PAGE 图谱显示几乎没有污染。免疫印迹分析表明，两种浓缩物中的 F. VIII 均可与 6 种针对 F. VIII 的单克隆抗体发生反应。这些结果表明，两种浓缩物中具有凝血活性的 F. VIII 分子的基本结构得以保留。