肺癌患者血液中循环DNA的RARβ2基因甲基化的MIRA分析

MIRA analysis of RARβ2 gene methylation in DNA circulating in the blood in lung cancer.

作者信息

Kapitskaya K Yu, Azhikina T L, Ponomaryova A A, Cherdyntseva N V, Vlasov V V, Laktionov P P, Rykova E Yu

机构信息

Institute of Organic Biochemistry, the Russian Academy of Sciences, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2014 Aug;157(4):516-9. doi: 10.1007/s10517-014-2604-z. Epub 2014 Aug 12.

DOI:10.1007/s10517-014-2604-z

PMID:25110096

Abstract

Analysis of DNA epigenetic mutations in the blood circulating DNA is a prospective trend for creation of noninvasive methods for the diagnosis and treatment efficiency monitoring in cancer. The methylation status of target genes in circulating DNA was evaluated by methods based on preliminary bisulfite conversion of DNA. We used a different approach based on selection of hypermethylated sequences of circulating DNA by means of DNA-methyl-binding protein (methylated CpG island recovery assay, MIRA). Methylation was evaluated for RARβ2 tumor suppression gene in circulating DNA in lung cancer and a trend was detected to higher methylation of this gene in the patients in comparison with healthy donors.

摘要

分析循环血液DNA中的表观遗传突变是创建癌症诊断及治疗效果监测无创方法的一个前瞻性趋势。基于DNA的亚硫酸氢盐预转化方法评估循环DNA中靶基因的甲基化状态。我们采用了一种不同的方法，即借助DNA甲基结合蛋白（甲基化CpG岛回收分析，MIRA）选择循环DNA的高甲基化序列。评估了肺癌患者循环DNA中RARβ2肿瘤抑制基因的甲基化情况，发现与健康供体相比，该基因在患者中的甲基化程度更高。