Kim Tong-Soo, Sohn Youngjoo, Kim Jung-Yeon, Lee Won-Ja, Na Byoung-kuk, Kang Yoon-Joong, Lee Hyeong-Woo
Department of Biomedical Science, Jungwon University, Goesan, Chungbuk 367-805, Republic of Korea.
Malar J. 2014 Aug 12;13:311. doi: 10.1186/1475-2875-13-311.
The purpose of this study was to examine the usefulness of the conserved block 9 (CB9) to interspecies conserved block (ICB10) region of Plasmodium vivax merozoite surface protein-1 (MSP-1 (ICB910)) as a serodiagnostic tool for understanding malaria transmission.
Antibody titre in the blood samples collected from the inhabitants of Gimpo city, Paju city and Yeoncheon county of Gyeonggi Province, as well as Cheorwon county of Gangwon Province, South Korea were determined by enzyme-linked immunosorbent assay (ELISA). Microscopic examination was performed to identify malarial parasites.
MSP-1(ICB910) is encoded by a 1,212-bp sequence, which produced a recombinant protein with a molecular weight of approximately 46 kDa. Antibody titres in 1,774 blood samples were determined with the help of ELISA using purified recombinant MSP-1(ICB910). The overall ELISA-positive rate was 8.08% (n = 146). The annual parasite incidences (APIs) in the regions where the blood sampling was carried out gradually decreased from 2004 to 2005 (1.09 and 0.80, respectively). Yeoncheon county had the highest ELISA-positive rate (10.20%, 46/451). Yeoncheon county also had the highest API both in 2004 and 2005, followed by Cheorwon county, Paju city and Gimpo city.
The MSP-1 (ICB910)-ELISA-positive rates were closely related to API in the geographic areas studied. These results suggest that sero-epidemiological studies employing MSP-1 (ICB910)-ELISA may be helpful in estimating the prevalence of malaria in certain geographic areas. MSP-1(ICB910)-ELISA can be effectively used to establish and evaluate malaria control and eradication programmes in the affected areas.
本研究旨在探讨间日疟原虫裂殖子表面蛋白1（MSP-1（ICB910））的保守区9（CB9）至种间保守区（ICB10）区域作为了解疟疾传播的血清学诊断工具的实用性。
采用酶联免疫吸附测定（ELISA）法测定从韩国京畿道金浦市、坡州市和涟川郡以及江原道铁原郡居民采集的血样中的抗体滴度。进行显微镜检查以鉴定疟原虫。
MSP-1（ICB910）由1212 bp的序列编码，产生一种分子量约为46 kDa的重组蛋白。使用纯化的重组MSP-1（ICB910）通过ELISA测定了1774份血样中的抗体滴度。ELISA总体阳性率为8.08%（n = 146）。采血地区的年寄生虫发病率（APIs）从2004年到2005年逐渐下降（分别为1.09和0.80）。涟川郡的ELISA阳性率最高（10.20%，46/451）。涟川郡在2004年和2005年的API也最高，其次是铁原郡、坡州市和金浦市。
在所研究的地理区域中，MSP-1（ICB910）-ELISA阳性率与API密切相关。这些结果表明，采用MSP-1（ICB910）-ELISA的血清流行病学研究可能有助于估计某些地理区域的疟疾流行情况。MSP-1（ICB910）-ELISA可有效地用于建立和评估受影响地区的疟疾控制和根除计划。
