用于检测新鲜农产品中弯曲杆菌属的选择性琼脂平板的研制。
Development of a selective agar plate for the detection of Campylobacter spp. in fresh produce.
作者信息
Yoo Jin-Hee, Choi Na-Young, Bae Young-Min, Lee Jung-Su, Lee Sun-Young
机构信息
Department of Food Science and Technology, Chung-Ang University, 72-1 Nae-ri, Daedeok-myeon, Anseong-si, Gyeonggi-do 456-756, South Korea.
Department of Food Science and Technology, Chung-Ang University, 72-1 Nae-ri, Daedeok-myeon, Anseong-si, Gyeonggi-do 456-756, South Korea.
出版信息
Int J Food Microbiol. 2014 Oct 17;189:67-74. doi: 10.1016/j.ijfoodmicro.2014.07.032. Epub 2014 Aug 2.
This study was conducted to develop a selective medium for the detection of Campylobacter spp. in fresh produce. Campylobacter spp. (n=4), non-Campylobacter (showing positive results on Campylobacter selective agar) strains (n=49) isolated from fresh produce, indicator bacteria (n=13), and spoilage bacteria isolated from fresh produce (n=15) were plated on four Campylobacter selective media. Bolton agar and modified charcoal cefoperazone deoxycholate agar (mCCDA) exhibited higher sensitivity for Campylobacter spp. than did Preston agar and Hunt agar, although certain non-Campylobacter strains isolated from fresh produce by using a selective agar isolation method, were still able to grow on Bolton agar and mCCDA. To inhibit the growth of non-Campylobacter strains, Bolton agar and mCCDA were supplemented with 5 antibiotics (rifampicin, polymyxin B, sodium metabisulfite, sodium pyruvate, ferrous sulfate) and the growth of Campylobacter spp. (n=7) and non-Campylobacter strains (n=44) was evaluated. Although Bolton agar supplemented with rifampicin (BR agar) exhibited a higher selectivity for Campylobacter spp. than did mCCDA supplemented with antibiotics, certain non-Campylobacter strains were still able to grow on BR agar (18.8%). When BR agar with various concentrations of sulfamethoxazole-trimethoprim were tested with Campylobacter spp. (n=8) and non-Campylobacter (n=7), sulfamethoxazole-trimethoprim was inhibitory against 3 of 7 non-Campylobacter strains. Finally, we validated the use of BR agar containing 50mg/L sulfamethoxazole (BRS agar) or 0.5mg/L ciprofloxacin (BRCS agar) and other selective agars for the detection of Campylobacter spp. in chicken and fresh produce. All chicken samples were positive for Campylobacter spp. when tested on mCCDA, BR agar, and BRS agar. In fresh produce samples, BRS agar exhibited the highest selectivity for Campylobacter spp., demonstrating its suitability for the detection of Campylobacter spp. in fresh produce.
本研究旨在开发一种用于检测新鲜农产品中弯曲杆菌属的选择性培养基。从新鲜农产品中分离出的弯曲杆菌属（n = 4）、非弯曲杆菌（在弯曲杆菌选择性琼脂上呈阳性结果）菌株（n = 49）、指示菌（n = 13）以及从新鲜农产品中分离出的腐败菌（n = 15）被接种到四种弯曲杆菌选择性培养基上。与普雷斯顿琼脂和亨特琼脂相比，博尔顿琼脂和改良的木炭头孢哌酮脱氧胆酸盐琼脂（mCCDA）对弯曲杆菌属表现出更高的敏感性，尽管通过选择性琼脂分离方法从新鲜农产品中分离出的某些非弯曲杆菌菌株仍能够在博尔顿琼脂和mCCDA上生长。为抑制非弯曲杆菌菌株的生长，在博尔顿琼脂和mCCDA中添加了5种抗生素（利福平、多粘菌素B、焦亚硫酸钠、丙酮酸钠、硫酸亚铁），并评估了弯曲杆菌属（n = 7）和非弯曲杆菌菌株（n = 44）的生长情况。尽管添加利福平的博尔顿琼脂（BR琼脂）对弯曲杆菌属的选择性高于添加抗生素的mCCDA，但某些非弯曲杆菌菌株仍能够在BR琼脂上生长（18.8%）。当用不同浓度的磺胺甲恶唑-甲氧苄啶对BR琼脂与弯曲杆菌属（n = 8）和非弯曲杆菌（n = 7）进行测试时，磺胺甲恶唑-甲氧苄啶对7株非弯曲杆菌菌株中的3株具有抑制作用。最后，我们验证了含50mg/L磺胺甲恶唑的BR琼脂（BRS琼脂）或0.5mg/L环丙沙星的BR琼脂（BRCS琼脂）以及其他选择性琼脂用于检测鸡肉和新鲜农产品中弯曲杆菌属的用途。所有鸡肉样品在mCCDA、BR琼脂和BRS琼脂上检测时，弯曲杆菌属均呈阳性。在新鲜农产品样品中，BRS琼脂对弯曲杆菌属表现出最高的选择性，证明其适用于检测新鲜农产品中的弯曲杆菌属。
Zotero 插件