热带臭虫（Cimex hemipterus，半翅目：臭虫科）中假定的kdr突变的鉴定

Identification of putative kdr mutations in the tropical bed bug, Cimex hemipterus (Hemiptera: Cimicidae).

作者信息

Dang Kai, Toi Cheryl S, Lilly David G, Lee Chow-Yang, Naylor Richard, Tawatsin Apiwat, Thavara Usavadee, Bu Wenun, Doggett Stephen L

机构信息

Department of Medical Entomology, Institute for Clinical Pathology and Medical Research, Westmead Hospital, Westmead, NSW, Australia.

Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, China.

出版信息

Pest Manag Sci. 2015 Jul;71(7):1015-20. doi: 10.1002/ps.3880. Epub 2014 Sep 11.

DOI:10.1002/ps.3880

PMID:25132449

Abstract

BACKGROUND

Bed bugs [both Cimex hemipterus (F.) and Cimex lectularius L.] are highly resistant to pyrethroids worldwide. An important resistance mechanism known as 'knockdown resistance' (kdr) is caused by genetic point mutations on the voltage-gated sodium channel (VGSC) gene. Previous studies have identified two point mutations (V419L and L925I) on the VGSC gene in C. lectularius that are responsible for kdr-type resistance. However, the kdr mutations in C. hemipterus have not been investigated.

RESULTS

Four novel mutations, L899V (leucine to valine), M918I (methionine to isoleucine), D953G (aspartic acid to glycine) and L1014F (leucine to phenylalanine), were identified in the domain II region of the C. hemipterus VGSC gene. This region has been widely investigated for the study of kdr-type resistance to pyrethroids in other insect pests. The V419L and L925I kdr mutations as previously identified in C. lectularius were not detected in C. hemipterus.

CONCLUSION

M918I and L1014F are considered to be probable kdr mutations and may play essential roles in kdr-type resistance to pyrethroids in C. hemipterus. Further studies are under way in the authors' laboratory to determine the non-kdr-type resistance mechanisms in C. hemipterus.

摘要

背景

臭虫（热带臭虫和温带臭虫）在全球范围内对拟除虫菊酯具有高度抗性。一种重要的抗性机制，即“击倒抗性”（kdr），是由电压门控钠通道（VGSC）基因上的基因点突变引起的。先前的研究已经在温带臭虫的VGSC基因上鉴定出两个导致kdr型抗性的点突变（V419L和L925I）。然而，热带臭虫中的kdr突变尚未得到研究。

结果

在热带臭虫VGSC基因的结构域II区域鉴定出四个新的突变，即L899V（亮氨酸突变为缬氨酸）、M918I（甲硫氨酸突变为异亮氨酸）、D953G（天冬氨酸突变为甘氨酸）和L1014F（亮氨酸突变为苯丙氨酸）。该区域已被广泛研究用于其他害虫对拟除虫菊酯的kdr型抗性研究。在热带臭虫中未检测到先前在温带臭虫中鉴定出的V419L和L925I kdr突变。