用于相关上转换发光和阴极发光成像的Y2O3:Tm,Yb纳米磷光体

Y2O3:Tm,Yb nanophosphors for correlative upconversion luminescence and cathodoluminescence imaging.

作者信息

Fukushima Shoichiro, Furukawa Taichi, Niioka Hirohiko, Ichimiya Masayoshi, Miyake Jun, Ashida Masaaki, Araki Tsutomu, Hashimoto Mamoru

机构信息

Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.

出版信息

Micron. 2014 Dec;67:90-95. doi: 10.1016/j.micron.2014.07.002. Epub 2014 Jul 18.

DOI:10.1016/j.micron.2014.07.002

PMID:25146422

Abstract

We present a phosphor nanoparticle that shows both upconversion luminescence (UCL) and cathodoluminescence (CL). With this particle, low-autofluorescence, deep-tissue and wide-field fluorescence imaging can be achieved with nanometer-order high-spatial-resolution imaging. We synthesized Y2O3:Tm,Yb nanophosphors that emit visible and near-infrared UCL under 980 nm irradiation and blue CL via electron beam excitation. The phosphors were applied to fluorescent imaging of HeLa cells. The photostability of the phosphors was superior to that of a conventional organic dye. We show that after uptake by HeLa cells, the particles can be imaged with SEM and CL contrast in a cellular section. This indicates that correlative UCL and CL imaging of biological samples could be realized.

摘要

我们展示了一种同时具有上转换发光（UCL）和阴极发光（CL）的磷光纳米颗粒。利用这种颗粒，可以实现低自发荧光、深层组织和宽视野荧光成像，并具有纳米级的高空间分辨率成像。我们合成了Y2O3:Tm,Yb纳米磷光体，其在980 nm照射下发射可见光和近红外UCL，并通过电子束激发产生蓝色CL。这些磷光体被应用于HeLa细胞的荧光成像。磷光体的光稳定性优于传统有机染料。我们表明，HeLa细胞摄取颗粒后，可以在细胞切片中通过扫描电子显微镜（SEM）和CL对比度进行成像。这表明可以实现生物样品的相关UCL和CL成像。