利用电化学金纳米粒子涂层丝网印刷免疫传感器检测食物中的 Ara h 1（一种主要的花生过敏原）。

Detection of Ara h 1 (a major peanut allergen) in food using an electrochemical gold nanoparticle-coated screen-printed immunosensor.

机构信息

REQUIMTE, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072 Porto, Portugal; REQUIMTE/Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.

REQUIMTE/Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.

出版信息

Biosens Bioelectron. 2015 Feb 15;64:19-24. doi: 10.1016/j.bios.2014.08.026. Epub 2014 Aug 19.

DOI:10.1016/j.bios.2014.08.026

PMID:25173734

Abstract

A gold nanoparticle-coated screen-printed carbon electrode was used as the transducer in the development of an electrochemical immunosensor for Ara h 1 (a major peanut allergen) detection in food samples. Gold nanoparticles (average diameter = 32 nm) were electrochemically generated on the surface of screen-printed carbon electrodes. Two monoclonal antibodies were used in a sandwich-type immunoassay and the antibody-antigen interaction was electrochemically detected through stripping analysis of enzymatically (using alkaline phosphatase) deposited silver. The total time of the optimized immunoassay was 3h 50 min. The developed immunosensor allowed the quantification of Ara h 1 between 12.6 and 2000 ng/ml, with a limit of detection of 3.8 ng/ml, and provided precise (RSD <8.7%) and accurate (recovery >96.6%) results. The immunosensor was successfully applied to the analysis of complex food matrices (cookies and chocolate), being able to detect Ara h 1 in samples containing 0.1% of peanut.

摘要

一种金纳米粒子修饰的丝网印刷碳电极被用作传感器，用于开发用于检测食品样品中 Ara h 1（主要的花生过敏原）的电化学免疫传感器。金纳米粒子（平均直径=32nm）通过电化学方法在丝网印刷碳电极的表面上生成。两种单克隆抗体被用于三明治型免疫分析，通过酶（使用碱性磷酸酶）沉积的银的剥落分析来电化学检测抗体-抗原相互作用。优化后的免疫分析总时间为 3 小时 50 分钟。所开发的免疫传感器允许在 12.6 至 2000ng/ml 之间定量检测 Ara h 1，检测限为 3.8ng/ml，提供了精确（RSD<8.7%）和准确（回收率>96.6%）的结果。该免疫传感器成功地应用于复杂食品基质（饼干和巧克力）的分析，能够检测到含有 0.1%花生的样品中的 Ara h 1。