[HPLC fingerprint of Callicarpa nudiflora].

OBJECTIVE

To establish HPLC fingerprint method for the quality evaluation and discrimination of Callicarpa nudiflora.

METHODS

The chromatographic fingerprint of the 12 samples was determined by injecting 20 microL of the sample solution each time on a reversed phase Agilent TC-C18 column with the gradient elution solvent system composed of 0.30% HAc solution and acetonitrile. The flow rate was 1.0 mL/min, the column temperature was maintained at 30 degrees C and the detection wavelength was set at 254 nm.

RESULTS

The HPLC fingerprint of Callicarpa nudiflora was set up,which included 20 common peaks, and the similarities of 12 samples were basically more than 0.90. Also,3 components were identified.

CONCLUSION

The method is accurate, repeatable, reliable and can fully reflect the chemical composition information, which can be used for the discrimination and the quality control of Callicarpa nudiflora.