[响应面法优化超细粉碎技术对人参须根总皂苷的溶出工艺]
[Optimization of dissolution process for superfine grinding technology on total saponins of Panax ginseng fibrous root by response surface methodology].
作者信息
Zhao Ya, Lai Xiao-Pin, Yao Hai-Yan, Zhao Ran, Wu Yi-Na, Li Geng
出版信息
Zhong Yao Cai. 2014 Mar;37(3):494-8.
OBJECTIVE
To investigate the effects of superfine comminution extraction technology of ginseng total saponins from Panax ginseng fibrous root, and to make sure the optimal extraction condition.
METHODS
Optimal condition of ginseng total saponins from Panax ginseng fibrous root was based on single factor experiment to study the effects of crushing degree, extraction time, alcohol concentration and extraction temperature on extraction rate. Response surface method was used to investigate three main factors such as superfine comminution time, extraction time and alcohol concentration.
RESULTS
The relationship between content of ginseng total saponins in Panax ginseng fibrous root and three factors fitted second degree polynomial models. The optimal extraction condition was 9 min of superfine comminution time, 70% of alcohol, 50 degrees C of extraction temperature and 70 min of extraction time. Under the optimal condition, ginseng total saponins from Panax ginseng fibrous root was average 94. 81%, which was consistent with the predicted value.
CONCLUSION
The optimization of technology is rapid, efficient, simple and stable.
目的
考察人参须根中人参总皂苷的超微粉碎提取工艺效果，确定最佳提取条件。
方法
在单因素实验基础上，以人参须根中人参总皂苷的最佳提取条件为研究对象，考察粉碎度、提取时间、乙醇浓度、提取温度对提取率的影响。采用响应面法考察超微粉碎时间、提取时间、乙醇浓度3个主要因素。
结果
人参须根中人参总皂苷含量与3个因素的关系拟合二次多项式模型。最佳提取条件为超微粉碎时间9 min、乙醇浓度70%、提取温度50℃、提取时间70 min。在此条件下，人参须根中人参总皂苷平均含量为94.81%，与预测值相符。
结论
该工艺优化快速、高效、简便、稳定。
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