人胚肾293细胞系在细胞生物学操作下的基因组动态变化
Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations.
作者信息
Lin Yao-Cheng, Boone Morgane, Meuris Leander, Lemmens Irma, Van Roy Nadine, Soete Arne, Reumers Joke, Moisse Matthieu, Plaisance Stéphane, Drmanac Radoje, Chen Jason, Speleman Frank, Lambrechts Diether, Van de Peer Yves, Tavernier Jan, Callewaert Nico
机构信息
1] Department of Plant Systems Biology, VIB, Technologiepark 927, Ghent B-9052, Belgium [2] Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, Ghent B-9052, Belgium [3].
1] Unit for Medical Biotechnology, Inflammation Research Center, VIB, Technologiepark 927, Ghent B-9052, Belgium [2] Laboratory for Protein Biochemistry and Biomolecular Engineering, Department of Biochemistry and Microbiology, Ghent University, Ledeganckstraat 35, Ghent B-9052, Belgium [3].
出版信息
Nat Commun. 2014 Sep 3;5:4767. doi: 10.1038/ncomms5767.
The HEK293 human cell lineage is widely used in cell biology and biotechnology. Here we use whole-genome resequencing of six 293 cell lines to study the dynamics of this aneuploid genome in response to the manipulations used to generate common 293 cell derivatives, such as transformation and stable clone generation (293T); suspension growth adaptation (293S); and cytotoxic lectin selection (293SG). Remarkably, we observe that copy number alteration detection could identify the genomic region that enabled cell survival under selective conditions (i.c. ricin selection). Furthermore, we present methods to detect human/vector genome breakpoints and a user-friendly visualization tool for the 293 genome data. We also establish that the genome structure composition is in steady state for most of these cell lines when standard cell culturing conditions are used. This resource enables novel and more informed studies with 293 cells, and we will distribute the sequenced cell lines to this effect.
HEK293人类细胞系在细胞生物学和生物技术中被广泛应用。在此，我们对六个293细胞系进行全基因组重测序，以研究这种非整倍体基因组在应对用于产生常见293细胞衍生物的操作时的动态变化，这些操作包括转化和稳定克隆生成（293T）；悬浮生长适应（293S）；以及细胞毒性凝集素选择（293SG）。值得注意的是，我们观察到拷贝数改变检测能够识别在选择性条件下（即蓖麻毒素选择）使细胞存活的基因组区域。此外，我们提出了检测人类/载体基因组断点的方法以及一个用户友好的293基因组数据可视化工具。我们还确定，当使用标准细胞培养条件时，这些细胞系中的大多数基因组结构组成处于稳定状态。这一资源有助于对293细胞进行新颖且更具信息性的研究，并且我们将为此分发已测序的细胞系。
Zotero 插件