Suppr超能文献

溶酶体相关细胞器复合体-1亚基1(BLOS1)与分选连接蛋白2及运输所需的内体分选复合体-I(ESCRT-I)组分TSG101相互作用,以介导表皮生长因子受体进入内体区室的分选过程。

Biogenesis of lysosome-related organelles complex-1 subunit 1 (BLOS1) interacts with sorting nexin 2 and the endosomal sorting complex required for transport-I (ESCRT-I) component TSG101 to mediate the sorting of epidermal growth factor receptor into endosomal compartments.

作者信息

Zhang Aili, He Xin, Zhang Ling, Yang Lin, Woodman Philip, Li Wei

机构信息

From the State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China, the University of Chinese Academy of Sciences, Beijing 100039, China, and.

From the State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

J Biol Chem. 2014 Oct 17;289(42):29180-94. doi: 10.1074/jbc.M114.576561. Epub 2014 Sep 2.

Abstract

Biogenesis of lysosome-related organelles complex-1 (BLOC-1) is a component of the molecular machinery required for the biogenesis of specialized organelles and lysosomal targeting of cargoes via the endosomal to lysosomal trafficking pathway. BLOS1, one subunit of BLOC-1, is implicated in lysosomal trafficking of membrane proteins. We found that the degradation and trafficking of epidermal growth factor receptor (EGFR) were delayed in BLOS1 knockdown cells, which were rescued through BLOS1 overexpression. A key feature to the delayed EGFR degradation is the accumulation of endolysosomes in BLOS1 knockdown cells or BLOS1 knock-out mouse embryonic fibroblasts. BLOS1 interacted with SNX2 (a retromer subunit) and TSG101 (an endosomal sorting complex required for transport subunit-I) to mediate EGFR lysosomal trafficking. These results suggest that coordination of the endolysosomal trafficking proteins is important for proper targeting of EGFR to lysosomes.

摘要

溶酶体相关细胞器复合体1(BLOC-1)的生物发生是通过内体到溶酶体运输途径进行特殊细胞器生物发生和货物溶酶体靶向所需分子机制的一个组成部分。BLOC-1的一个亚基BLOS1与膜蛋白的溶酶体运输有关。我们发现,在BLOS1基因敲低的细胞中,表皮生长因子受体(EGFR)的降解和运输延迟,而通过BLOS1过表达可使其恢复。EGFR降解延迟的一个关键特征是在BLOS1基因敲低的细胞或BLOS1基因敲除的小鼠胚胎成纤维细胞中内溶酶体的积累。BLOS1与SNX2(一种回收复合体亚基)和TSG101(运输所需的内体分选复合体亚基I)相互作用,介导EGFR的溶酶体运输。这些结果表明,内溶酶体运输蛋白的协调对于EGFR正确靶向溶酶体很重要。

相似文献

3
Endosomal type Iγ PIP 5-kinase controls EGF receptor lysosomal sorting.
Dev Cell. 2013 Apr 29;25(2):144-55. doi: 10.1016/j.devcel.2013.03.010. Epub 2013 Apr 18.
4
RAB7 and TSG101 are required for the constitutive recycling of unliganded EGFRs via distinct mechanisms.
Mol Cell Endocrinol. 2013 Dec 5;381(1-2):188-97. doi: 10.1016/j.mce.2013.07.029. Epub 2013 Aug 7.
5
Cargo-dependent degradation of ESCRT-I as a feedback mechanism to modulate endosomal sorting.
Traffic. 2011 Sep;12(9):1211-26. doi: 10.1111/j.1600-0854.2011.01220.x. Epub 2011 Jun 13.
6
The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor.
Mol Biol Cell. 2006 Jun;17(6):2513-23. doi: 10.1091/mbc.e05-10-0915. Epub 2006 Mar 22.
7
Recognising the signals for endosomal trafficking.
Curr Opin Cell Biol. 2020 Aug;65:17-27. doi: 10.1016/j.ceb.2020.02.005. Epub 2020 Mar 7.
9
BLOS1, a putative BLOC-1 subunit, interacts with SNX1 and modulates root growth in Arabidopsis.
J Cell Sci. 2010 Nov 1;123(Pt 21):3727-33. doi: 10.1242/jcs.069732.

引用本文的文献

1
BLOC-1 and BORC: Complex regulators of endolysosomal dynamics.
Cell Chem Biol. 2025 Aug 26. doi: 10.1016/j.chembiol.2025.08.001.
4
Hermansky-Pudlak Syndrome: From Molecular Pathogenesis to Targeted Therapies.
IUBMB Life. 2025 May;77(5):e70025. doi: 10.1002/iub.70025.
5
BLOC1S1 control of vacuolar organelle fidelity modulates T2 cell immunity and allergy susceptibility.
bioRxiv. 2024 Mar 27:2024.03.21.586144. doi: 10.1101/2024.03.21.586144.
9
The endo-lysosomal regulatory protein BLOC1S1 modulates hepatic lysosomal content and lysosomal lipolysis.
Biochem Biophys Res Commun. 2023 Jan 29;642:1-10. doi: 10.1016/j.bbrc.2022.12.038. Epub 2022 Dec 14.

本文引用的文献

1
Dysbindin-1C is required for the survival of hilar mossy cells and the maturation of adult newborn neurons in dentate gyrus.
J Biol Chem. 2014 Oct 17;289(42):29060-72. doi: 10.1074/jbc.M114.590927. Epub 2014 Aug 25.
2
Mutations in the BLOC-1 subunits dysbindin and muted generate divergent and dosage-dependent phenotypes.
J Biol Chem. 2014 May 16;289(20):14291-300. doi: 10.1074/jbc.M114.553750. Epub 2014 Apr 8.
3
Atg5 regulates late endosome and lysosome biogenesis.
Sci China Life Sci. 2014 Jan;57(1):59-68. doi: 10.1007/s11427-013-4588-8. Epub 2013 Dec 23.
4
5
Statistical tests for measures of colocalization in biological microscopy.
J Microsc. 2013 Dec;252(3):295-302. doi: 10.1111/jmi.12093. Epub 2013 Oct 10.
6
8
Hermansky-Pudlak syndrome: pigmentary and non-pigmentary defects and their pathogenesis.
Pigment Cell Melanoma Res. 2013 Mar;26(2):176-92. doi: 10.1111/pcmr.12051. Epub 2012 Dec 31.
10
The BLOS1-interacting protein KXD1 is involved in the biogenesis of lysosome-related organelles.
Traffic. 2012 Aug;13(8):1160-9. doi: 10.1111/j.1600-0854.2012.01375.x. Epub 2012 May 28.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验