开发唇形科香茶菜属长管香茶菜的微卫星标记。
Development of microsatellite markers for Isodon longitubus (Lamiaceae).
机构信息
Environmental Symbiosis Studies, Graduate School of Integrated Arts and Sciences, University of Tokushima, 1-1 Minami-josanjima, Tokushima 770-8502, Japan.
Graduate School of Advanced Technology and Science, University of Tokushima, 2-1 Minami-josanjima, Tokushima 770-8506, Japan.
出版信息
Appl Plant Sci. 2013 Oct 1;1(10). doi: 10.3732/apps.1300028. eCollection 2013 Oct.
PREMISE OF THE STUDY
Microsatellite markers were developed for Isodon longitubus to study the natural hybridization of the species and its congeners. •
METHODS AND RESULTS
A total of 10 primer sets were developed for I. longitubus. From the initial screening, all of 10 loci were polymorphic with five to 19 alleles per locus in the Mt. Ishizuchi population, whereas nine loci were polymorphic with two to 12 alleles per loci in the Toon population. Although one locus was monomorphic at one population, the observed and expected heterozygosity values estimated from 34 I. longitubus samples ranged from 0.273 to 1.000 and from 0.483 to 0.918, respectively. Six primer sets could amplify all three species examined in this study (I. inflexus, I. japonicus, and I. shikokianus). •
CONCLUSIONS
The 10 microsatellite markers developed here will be useful in analyzing the population genetic structure of I. longitubus and in studying the natural hybridization between Isodon species.
研究前提
为了研究该物种及其近缘种的自然杂交，开发了 Isodon longitubus 的微卫星标记。
方法与结果
共为 I. longitubus 开发了 10 对引物。从初步筛选结果来看，在 Mt. Ishizuchi 种群中，所有 10 个位点均具有多态性，每个位点有 5 到 19 个等位基因；而在 Toon 种群中，9 个位点具有多态性，每个位点有 2 到 12 个等位基因。尽管一个位点在一个种群中是单态的，但从 34 个 I. longitubus 样本中估计的观察和预期杂合度值分别在 0.273 到 1.000 和 0.483 到 0.918 之间。有 6 对引物可以扩增本研究中检查的所有三个物种（I. inflexus、I. japonicus 和 I. shikokianus）。
结论
本研究开发的 10 个微卫星标记将有助于分析 I. longitubus 的种群遗传结构，并研究 Isodon 物种之间的自然杂交。
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