肾脏和睾丸肿瘤中的内源性生物素表达及文献综述
Endogenous biotin expression in renal and testicular tumours and literature review.
作者信息
Fahmy Nader, Woo Mark, Alameldin Mona, Lee Joe King, MacDonald Kyle, Goneau Lee W, Cadieux Peter, Burton Jeremy, Pautler Stephen
机构信息
Division of Urology, Department of Surgery, Western University, London ON;
Department of Anatomical Pathology, Western University, London ON;
出版信息
Can Urol Assoc J. 2014 Jul;8(7-8):268-72. doi: 10.5489/cuaj.1810.
INTRODUCTION
The aim of this study was to examine endogenous biotin levels in tumour specimens collected from patients with renal and testicular tumours and compare them to the surrounding non-neoplastic surgical margin.
METHODS
Frozen samples were obtained from the Ontario Tumour Bank. Renal and testicular tumour tissue were included in this study. Normal tissue from the negative surgical margins of each tumour served as a control. Biotin detection in tissue specimens was determined using immunohistochemistry (IHC).
RESULTS
Specimens collected from 56 patients (36 men and 20 women) were included in this study. Histopathology of the 52 renal tumours included 31 (60%) conventional type RCC, 5 (10%) chromophobe RCC, 5 (10%) papillary RCC, 1 (2%) oncocytoma and 10 (19%) upper tract urothelial carcinoma (UC). The 4 testicular tumours included 1 seminomatous (25%) germ cell tumour and 3 (75%) non seminomatous germ cell tumours.
CONCLUSION
No biotin signal was perceived in all tested tumour samples. Endogenous biotin expression was detected in the matching non-neoplastic surgical margin of tested renal tissues. This lack of staining may prove to be a valuable tool in future studies.
引言
本研究旨在检测从肾肿瘤和睾丸肿瘤患者采集的肿瘤标本中的内源性生物素水平，并将其与周围非肿瘤性手术切缘进行比较。
方法
从安大略肿瘤库获取冷冻样本。本研究纳入了肾肿瘤和睾丸肿瘤组织。每个肿瘤阴性手术切缘的正常组织作为对照。使用免疫组织化学（IHC）测定组织标本中的生物素。
结果
本研究纳入了56例患者（36例男性和20例女性）的标本。52例肾肿瘤的组织病理学类型包括31例（60%）传统型肾细胞癌、5例（10%）嫌色性肾细胞癌、5例（10%）乳头状肾细胞癌、1例（2%）嗜酸细胞瘤和10例（19%）上尿路尿路上皮癌（UC）。4例睾丸肿瘤包括1例（25%）精原细胞瘤性生殖细胞肿瘤和3例（75%）非精原细胞瘤性生殖细胞肿瘤。
结论
在所有检测的肿瘤样本中均未检测到生物素信号。在检测的肾组织匹配的非肿瘤性手术切缘中检测到内源性生物素表达。这种缺乏染色可能在未来研究中被证明是一种有价值的工具。
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