实验性肺炎球菌性脑膜炎中脑实质肿瘤坏死因子-α和白细胞介素-1β的诱导
Brain parenchymal TNF-α and IL-1β induction in experimental pneumococcal meningitis.
作者信息
Izadpanah Kaywan, Freyer Dorette, Weber Joerg R, Braun Johann S
机构信息
Department of Neurology, Charité Universitätsmedizin Berlin, Germany; Department of Orthopedics and Traumatology, University Freiburg, Germany.
Department of Neurology, Charité Universitätsmedizin Berlin, Germany.
出版信息
J Neuroimmunol. 2014 Nov 15;276(1-2):104-11. doi: 10.1016/j.jneuroim.2014.08.625. Epub 2014 Sep 2.
Triggers of brain inflammation in pneumococcal meningitis are unknown. TNF-α and IL-1β were upregulated (real time PCR and in situ hybridization) in neurons and astrocytes time-dependently and maximally in the hippocampus during murine pneumococcal meningitis. Upregulation of TNF-α and IL-1β mRNA in the brain parenchyma was independent of cerebrospinal fluid leukocytosis, pneumococcal pneumolysin and H2O2, but it was potently induced by pneumococcal cell wall (PCW) fragments. Brain TNF-α mRNA was downregulated by a matrix metalloproteinases inhibitor. PCW fragments were located in the brain parenchyma. In conclusion, PCW fragments and matrix metalloproteinases trigger cytokine induction in the brain parenchyma during pneumococcal meningitis.
肺炎球菌性脑膜炎中脑炎症的触发因素尚不清楚。在小鼠肺炎球菌性脑膜炎期间，肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）在神经元和星形胶质细胞中呈时间依赖性上调（实时聚合酶链反应和原位杂交），且在海马体中上调最为明显。脑实质中TNF-α和IL-1β信使核糖核酸（mRNA）的上调与脑脊液白细胞增多、肺炎球菌溶血素和过氧化氢无关，但肺炎球菌细胞壁（PCW）片段可强烈诱导其上调。脑TNF-α mRNA被一种基质金属蛋白酶抑制剂下调。PCW片段位于脑实质中。总之，PCW片段和基质金属蛋白酶在肺炎球菌性脑膜炎期间触发脑实质中的细胞因子诱导。
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