Pettersson A, Hedner J, Hedner T
Department of Pharmacology and Clinical Pharmacology, Gothenburg University, Sweden.
Acta Physiol Scand. 1989 Apr;135(4):487-92. doi: 10.1111/j.1748-1716.1989.tb08607.x.
The diuretic and natriuretic effects of r-alpha-ANP (99-126) were investigated in rats with chronic ischaemic heart failure (IHF) produced by left coronary artery ligation. The plasma concentration of immunoreactive ANP (IrANP) was significantly higher, 91.8 +/- 16.0 pm in the IHF rats compared to 31.0 +/- 4.9 pm in sham-operated controls. In the control rats, ANP infusion (0.25-1.0 micrograms kg 1 mm 1) increased urine flow rate (V) and urinary sodium (UNa V) excretion. At the highest dose level, both V and UNa V were increased approximately fivefold. The diuresis and natriuresis seen in the control group after the infusion of ANP were blunted in the IHF rats. A bilateral surgical renal denervation in the IHF rats did not alter the renal dopamine levels, but induced a significant decrease in renal noradrenaline content, and almost completely restored the renal responsiveness to the ANP infusions. We conclude that renal denervation reversed the blunted renal excretory response to ANP in IHF rats. Thus, in experimental IHF, there seems to be a functional antagonism between efferent renal sympathetic nerve activity and ANP.
在通过左冠状动脉结扎产生慢性缺血性心力衰竭（IHF）的大鼠中，研究了r-α-ANP（99-126）的利尿和排钠作用。免疫反应性ANP（IrANP）的血浆浓度在IHF大鼠中显著更高，为91.8±16.0皮摩尔，而假手术对照组为31.0±4.9皮摩尔。在对照大鼠中，输注ANP（0.25-1.0微克/千克·分钟）可增加尿流率（V）和尿钠（UNa V）排泄。在最高剂量水平时，V和UNa V均增加约五倍。在IHF大鼠中，输注ANP后对照组中出现的利尿和排钠作用减弱。对IHF大鼠进行双侧手术去肾神经支配并未改变肾多巴胺水平，但导致肾去甲肾上腺素含量显著降低，并且几乎完全恢复了肾脏对输注ANP的反应性。我们得出结论，去肾神经支配逆转了IHF大鼠中对ANP减弱的肾排泄反应。因此，在实验性IHF中，肾传出交感神经活动与ANP之间似乎存在功能拮抗作用。
