印度东南海岸恩诺尔河口有毒金属诱导的海洋浮游动物遗传毒性监测。
Monitoring of genotoxicity in marine zooplankton induced by toxic metals in Ennore estuary, Southeast coast of India.
机构信息
Unit of Aquaculture and Cryobiology, Department of Zoology, University of Madras, Guindy Campus, Chennai 600025, India.
UGC Academic Staff College, University of Madras, Chepauk Campus, Chennai 600005, India.
出版信息
Mar Pollut Bull. 2014 Nov 15;88(1-2):70-80. doi: 10.1016/j.marpolbul.2014.09.025. Epub 2014 Oct 3.
The present study provides preliminary in-situ data on genetic integrity of marine zooplankton. Paracalanus parvus, Oithona rigida and Euterpina acutifrons were collected during four different seasons (summer, pre-monsoon, monsoon and post-monsoon) from 2011 to 2012 in Ennore and Kovalum estuaries. DNA damage levels in different zooplankton were analyzed by comet assay and were correlated with different environmental stressors. Spatial and temporal variations in DNA damage was observed in all the species. Zooplankton from Ennore estuary showed significantly lower genetic integrity. Particulate, sediment, and zooplankton fractions of Pb, Ni, Cu, Cr and Co were associated with high DNA damage during the period of lowest pH, salinity and dissolved oxygen. Zn and Cd showed lower genotoxic impact than the other metals. Feeding modes strongly influenced the genetic integrity in the zooplankton species studied. These results support the use of comet assay as a tool in effectively monitoring genotoxicity in marine plankton communities.
本研究提供了海洋浮游动物遗传完整性的初步原位数据。从 2011 年到 2012 年，在恩诺尔和科瓦鲁姆河口的四个不同季节（夏季、前季风期、季风期和后季风期）采集了小拟哲水蚤、秀丽白虾和真刺唇角水蚤。通过彗星试验分析了不同浮游动物的 DNA 损伤水平，并将其与不同的环境胁迫因素相关联。在所有物种中都观察到了 DNA 损伤的时空变化。恩诺尔河口的浮游动物表现出明显较低的遗传完整性。在 pH 值、盐度和溶解氧最低的时期，浮游动物、沉积物和颗粒体中 Pb、Ni、Cu、Cr 和 Co 与高 DNA 损伤有关。Zn 和 Cd 比其他金属的遗传毒性影响较小。摄食方式强烈影响了所研究浮游动物物种的遗传完整性。这些结果支持使用彗星试验作为一种有效监测海洋浮游动物群落遗传毒性的工具。
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