与κ阿片受体（KOPR）相互作用的蛋白质的鉴定与验证。
Identification and verification of proteins interacting with the kappa opioid receptor (KOPR).
作者信息
Chen Chongguang, Huang Peng, Liu-Chen Lee-Yuan
机构信息
Center for Substance Abuse Research and Department of Pharmacology, Temple University School of Medicine, 3500 N. Broad Street, MERB 851, Philadelphia, PA, 19140, USA.
出版信息
Methods Mol Biol. 2015;1230:129-40. doi: 10.1007/978-1-4939-1708-2_10.
Proteins that interact with the human kappa opioid receptor (hKOPR) may contribute to regulation and signaling of the receptor. In this paper, we focus on the protein 14-3-3zeta that regulates anterograde transport of the hKOPR from the endoplasmic reticulum (ER) to the Golgi apparatus. 14-3-3zeta interacts with the C-terminal domain of the receptor and promotes cell surface expression of the hKOPR by inhibiting coatomer protein I (COPI) and RVR motif-mediated ER retension of the hKOPR. Here we describe three experimental procedures we used to evaluate the interaction between hKOPR and 14-3-3zeta: co-immunoprecipitation, pull-down assay and immunofluorescence microscopy.
与人类κ阿片受体（hKOPR）相互作用的蛋白质可能有助于该受体的调节和信号传导。在本文中，我们聚焦于调节hKOPR从内质网（ER）到高尔基体的顺向转运的蛋白质14-3-3ζ。14-3-3ζ与该受体的C末端结构域相互作用，并通过抑制外衣蛋白I（COPI）和RVR基序介导的hKOPR的内质网滞留来促进hKOPR的细胞表面表达。在此，我们描述了用于评估hKOPR与14-3-3ζ之间相互作用的三个实验程序：免疫共沉淀、下拉测定和免疫荧光显微镜检查。
Zotero 插件