从乌墨（Eugenia jambolana Lam.）中分离出的内生真菌的体外抗氧化活性和总酚含量

In vitro antioxidant activity and total phenolic content of endophytic fungi isolated from Eugenia jambolana Lam.

作者信息

Yadav Manila, Yadav Amita, Yadav Jaya Parkash

机构信息

Department of Genetics, M.D. University, Rohtak-124001, Haryana, India.

出版信息

Asian Pac J Trop Med. 2014 Sep;7S1:S256-61. doi: 10.1016/S1995-7645(14)60242-X.

DOI:10.1016/S1995-7645(14)60242-X

PMID:25312132

Abstract

OBJECTIVE

To elucidate the antioxidant activity and total phenolic content (TPC) of ethyl acetate extracts of endophytic fungi isolated from Eugenia jambolana by three different antioxidant assays.

METHODS

Twenty one different endophytic fungal extracts were screened for presence of various phytochemicals, TPC and in vitro antioxidant activity. TPC was tested by Folin-Ciocalteau reagent based assay. DPPH free radical scavenging, hydrogen peroxide scavenging and reducing power assays were used to evaluate the antioxidant activity.

RESULTS

Alkaloids, phenols, flavonoids, saponins, and terpenes were the main phytochemicals presents in all 21 endophytes. A significant positive correlation was found between antioxidant activity and TPC in fungal extracts. There is 36% endophytic extracts having high phenolic content exhibited potent antioxidant activity. Chaetomium sp., Aspergillus sp., Aspergillus peyronelii and Aspergillus niger strain showed the highest antioxidant activity ranging from 50% to 80% having 58 mg/g to 60 mg/g GAE total phenolics. Ascorbic acid used as a standard showed 90% reducing potential.

CONCLUSIONS

The results reveal that metabolites produced by endophytic fungi isolated from Eugenia jambolana can be a potential source of novel natural antioxidant compounds.

摘要

目的

通过三种不同的抗氧化测定方法，阐明从蒲桃中分离出的内生真菌乙酸乙酯提取物的抗氧化活性和总酚含量（TPC）。

方法

对21种不同的内生真菌提取物进行筛选，检测各种植物化学物质的存在、TPC和体外抗氧化活性。通过基于福林-西奥尔特试剂的测定法检测TPC。采用DPPH自由基清除、过氧化氢清除和还原能力测定法评估抗氧化活性。

结果

生物碱、酚类、黄酮类、皂苷类和萜类是所有21种内生真菌中主要的植物化学物质。在真菌提取物中，抗氧化活性与TPC之间存在显著的正相关。36%的高酚含量内生真菌提取物表现出较强的抗氧化活性。毛壳菌属、曲霉属、佩罗内曲霉和黑曲霉菌株表现出最高的抗氧化活性，范围为50%至80%，总酚含量为58 mg/g至60 mg/g没食子酸当量。用作标准的抗坏血酸显示出90%的还原潜力。