刺山柑不同部位提取物的体外抗氧化潜力比较分析

A comparative in vitro antioxidant potential profile of extracts from different parts of Fagonia cretica.

作者信息

Iqbal Pervaiz, Ahmed Dildar, Asghar Muhammad Nadeem

机构信息

Department of Chemistry, Forman Christian College (A Chartered University), Lahore 54600, Pakistan.

出版信息

Asian Pac J Trop Med. 2014 Sep;7S1:S473-80. doi: 10.1016/S1995-7645(14)60277-7.

DOI:10.1016/S1995-7645(14)60277-7

PMID:25312170

Abstract

OBJECTIVE

To evaluate antioxidant and radical scavenging activities of organic extracts from fruit, roots and aerial parts of Fagonia cretica.

METHODS

Shed dried and powdered plant parts were initially extracted in methanol and subsequently partitioned in n-hexane, chloroform, ethyl acetate and 1-butanol successively. Antioxidant and radical scavenging potential of the methanol extracts and the fractions of each part were evaluated using total phenolic contents (TPC) and total flavonoid contents (TFC), 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation radicals scavenging, reducing power (potassium ferricyanide-trichloroacetic acid system), ferric ion reducing antioxidant potential, lipid peroxidation inhibition activity (linoleic acid system) and total antioxidant activity (phosphomolybdate) assays.

RESULTS

TPC and TFC values for methanol extracts and various fractions ranged from 0.23-4.30 mg/L gallic acid equivalents and from 30-545 mg/L rutin equivalents, respectively. Overall, methanol extracts and all the fractions of root and aerial parts showed higher TPC and TFC values. Methanol extracts and aqueous fractions of root and aerial parts and the n-butanol fraction of root showed lower EC50 values for 2,2-diphenyl-1-picrylhydrazyl scavenging than the other plant extracts. The 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging, total antioxidant potential and ferric ion reducing antioxidant potential values confirmed the presence of potent antioxidant principles in the methanol extract of roots. In general, all the extracts/fractions and especially those of root showed high antioxidant and radical scavenging activities.

CONCLUSIONS

The crude methanol extract of root can be explored further for in vivo studies. This study revealed the potent antioxidant potential of Fagonia cretica and its prospective efficacy against various reactive oxygen species-mediated diseases.

摘要

目的

评估刺山柑果实、根及地上部分有机提取物的抗氧化和自由基清除活性。

方法

将干燥并粉碎的植物部分先用甲醇提取，随后依次用正己烷、氯仿、乙酸乙酯和正丁醇进行分配。使用总酚含量（TPC）、总黄酮含量（TFC）、2,2-二苯基-1-苦基肼自由基和2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）自由基阳离子自由基清除、还原能力（铁氰化钾-三氯乙酸体系）、铁离子还原抗氧化能力、脂质过氧化抑制活性（亚油酸体系）和总抗氧化活性（磷钼酸盐）测定法评估甲醇提取物及各部分馏分的抗氧化和自由基清除潜力。

结果

甲醇提取物和各种馏分的TPC值和TFC值分别为0.23 - 4.30毫克/升没食子酸当量和30 - 545毫克/升芦丁当量。总体而言，甲醇提取物以及根和地上部分的所有馏分显示出较高的TPC和TFC值。根和地上部分的甲醇提取物及水相馏分以及根的正丁醇馏分在2,2-二苯基-1-苦基肼清除方面的半数有效浓度（EC50）值低于其他植物提取物。2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）自由基清除、总抗氧化能力和铁离子还原抗氧化能力值证实根的甲醇提取物中存在有效的抗氧化成分。一般来说，所有提取物/馏分，尤其是根的提取物/馏分显示出高抗氧化和自由基清除活性。