Shieh Peyton, Bertozzi Carolyn R
Department of Chemistry, University of California, Berkeley, California 94720, USA.
Org Biomol Chem. 2014 Dec 14;12(46):9307-20. doi: 10.1039/c4ob01632g. Epub 2014 Oct 15.
Bioorthogonal chemistry has enabled the selective labeling and detection of biomolecules in living systems. Bioorthogonal smart probes, which become fluorescent or deliver imaging or therapeutic agents upon reaction, allow for the visualization of biomolecules or targeted delivery even in the presence of excess unreacted probe. This review discusses the strategies used in the development of bioorthogonal smart probes and highlights the potential of these probes to further our understanding of biology.
生物正交化学能够在生物系统中对生物分子进行选择性标记和检测。生物正交智能探针在反应后会发出荧光或递送成像或治疗剂,即使在存在过量未反应探针的情况下,也能实现生物分子的可视化或靶向递送。本文综述了生物正交智能探针开发中使用的策略,并强调了这些探针在加深我们对生物学理解方面的潜力。