Non-scan and real-time multichannel angular surface plasmon resonance imaging method.

We describe a non-scan and real-time multichannel angular surface plasmon resonance (SPR) imaging method. We demonstrate experimentally, with multiple line shaped light illuminations to construct multi-imaging channels, that an image captured with an area detector can probe the surface plasmons with different field distributions. Thus, it provides a fixed optical-sensing module measuring the spatial variations of the refractive index in the flow system in real time without scanning. This technique has the advantages of high system stability and similar optical arrangement to the conventional parallel-channel SPR sensors and could have potential applications in multianalyte detection and reference-compensated biosensing.