虎眼万年青的甲醇提取物诱导PC-3前列腺癌细胞系和WEHI-164纤维肉瘤癌细胞系凋亡。
Methanolic Fractions of Ornithogalum cuspidatum Induce Apoptosis in PC-3 Prostate Cancer Cell Line and WEHI-164 Fibrosarcoma Cancer Cell Line.
作者信息
Asadi Hamed, Orangi Mona, Shanehbandi Dariush, Babaloo Zohreh, Delazar Abbas, Mohammadnejad Leila, Zare Shahneh Fatemeh, Valiyari Samira, Baradaran Behzad
机构信息
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Adv Pharm Bull. 2014 Oct;4(Suppl 1):455-8. doi: 10.5681/apb.2014.067. Epub 2014 Aug 25.
PURPOSE
The present study, was aimed to assess the cytotoxic effects of Ornithogalum cuspidatum methanolic fractions on PC-3, prostate cancer cells and WEHI-164, Fibrosarcoma cells.
METHODS
Methanolic fractions of O. cuspidatum were prepared using solid phase extraction and the cells were treated with different concentrations for 12 and 24 hours. Cytotoxicity and cell viability were measured by MTT assay. ELISA was also employed to assess the histone-associated DNA fragments and the involvement of apoptotic mechanisms.
RESULTS
10 and 20% fractions had not significant cytotoxic effects (p>0.05) but other fractions exerted growth inhibition on both cancer cell lines (p<0.05). After 24h of incubation with 40, 60, 80 and 100% fractions, the IC50 values were: 165, 85, 65 and 45μg/ml on PC-3 cells and 200, 96, 76 and 73μg/ml against WEHI-164 cell line, respectively. ELISA results also revealed that, both cell lines had undergone apoptosis.
CONCLUSION
It is deduced that, 80% and 100% methanolic fractions had significant anti-proliferative and apoptotic impacts on PC-3 and WEHI-164 cells in vitro and could be considered for developing chemo-preventive substances.
目的
本研究旨在评估虎眼万年青甲醇提取物对前列腺癌细胞PC - 3和纤维肉瘤细胞WEHI - 164的细胞毒性作用。
方法
采用固相萃取法制备虎眼万年青甲醇提取物，并用不同浓度提取物处理细胞12小时和24小时。通过MTT法检测细胞毒性和细胞活力。还采用ELISA法评估组蛋白相关DNA片段及凋亡机制的参与情况。
结果
10%和20%的提取物无显著细胞毒性作用（p>0.05），但其他提取物对两种癌细胞系均有生长抑制作用（p<0.05）。用40%、60%、80%和100%的提取物孵育24小时后，PC - 3细胞的IC50值分别为165、85、65和45μg/ml，对WEHI - 164细胞系的IC50值分别为200、96、76和73μg/ml。ELISA结果还显示，两种细胞系均发生了凋亡。
结论
推断80%和100%的甲醇提取物在体外对PC - 3和WEHI - 164细胞具有显著的抗增殖和凋亡作用，可考虑用于开发化学预防物质。
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